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Through training, the investigators can adjust the composition and functions of gut microbiota. Exercise also alters the body's energy metabolism pathways, which, in turn, impacts the gut microbiota and its metabolic products. Understanding how the body utilizes and regulates energy during exercise is essential for enhancing athletic performance. This study aims to explore the relationships among post-exercise energy metabolites, gut microbiota, and their metabolic products, applying omics technologies in the field of sports science.
This interdisciplinary research will contribute to fields such as exercise physiology, sports nutrition, exercise biochemistry, and sports medicine, providing innovative and practical research insights for advancing sports science in our country.
The gut microbiota, which is comprised of trillions of microbes, is the collective term for the gut microbiota. It establishes a symbiotic relationship with the host and plays a vital role in maintaining host health. Through training, the investigators can adjust the composition and functions of gut microbiota. Exercise also alters the body's energy metabolism pathways, which, in turn, impacts the gut microbiota and its metabolic products. Understanding how the body utilizes and regulates energy during exercise is essential for enhancing athletic performance. This study aims to explore the relationships among post-exercise energy metabolites, gut microbiota, and their metabolic products, applying omics technologies in the field of sports science.
This interdisciplinary research will contribute to fields such as exercise physiology, sports nutrition, exercise biochemistry, and sports medicine, providing innovative and practical research insights for advancing sports science in our country.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Marathon running group | Experimental | Run 10 kilometers on a treadmill. |
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| General healthy adult group | No Intervention | No intervention will be conducted. |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| running | Device | Running 10 kilometers on a treadmill |
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| Measure | Description | Time Frame |
|---|---|---|
| Concentration of lactate | Measure changes in lactate concentration. | The testing time points are set as follows: before exercise, at the 5-kilometer mark during exercise, immediately after exercise, and at 5, 15, 30, and 60 minutes post-exercise. |
| Concentration of creatine kinase | Measure changes in creatine kinase concentration. | The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise. |
| Concentration of glucose | Measure changes in glucose concentration. | The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise. |
| Concentration of creatinine | Measure changes in creatinine concentration. | The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise. |
| Concentration of blood urea nitrogen | Measure changes in blood urea nitrogen concentration. | The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise. |
| Concentration of lactate dehydrogenase | Measure changes in lactate dehydrogenase concentration. | The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise. |
| Concentration of free fatty acid |
| Measure | Description | Time Frame |
|---|---|---|
| Body composition | The investigators used the InBody 570 (In-body, Seoul, South Korea), the bioelectrical impedance analyzer (BIA) to measure body composition. The device can screen frequencies of 1, 5, 50, 260, 500 and 1000 kHz within 60 seconds. After cleaning the palms and soles of the feet, the subject stood vertically on the electrodes of the instrument, holding the sensing handle with both hands and keeping the arms away from the body at a 30° angle, and avoided talking or moving during the measurement. The device measured body weight (kg), BMI (kg/m²), fat mass (%), and lean mass (kg). |
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Marathon Running Group
Inclusion Criteria:
Exclusion Criteria:
General Healthy Adult Group
Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Yi-Ju Hsu | Contact | +886-3-328-3201 #2430 | ruby780202@ntsu.edu.tw |
| Name | Affiliation | Role |
|---|---|---|
| Yi-Ju Hsu | Associate Professor | Principal Investigator |
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Measure changes in free fatty acid concentration. |
| The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise. |
| Concentration of cortisol | Measure changes in cortisol concentration. | The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise. |
| ood frequency questionnaire(FFQ) | The participants' diets were assessed using a semiquantitative food frequency questionnaire(FFQ). The FFQ used in this study was validated in a previous study; the questionnaire exhibited reliability and validity for identifying major nutrients in the diets of Taiwanese vegetarians and omnivores.(Chiu TH et al., 2014) In this study, the nutrient analysis programme used to calculate the results of the FFQ was based on Taiwan's Food Composition Database. To assess the intake frequency of each food item, frequencies ranged from "never" or "<1 time per month" to "more than 2 times per day. | Before exercise |
| Gut microbiota analysis | Collect participant stool samples for gut microbiota analysis. | The testing time points are set as follows: before exercise, immediately after exercise, and at 24 and 48 hours post-exercise. |
| Untargeted metabolome analysis | Collect participant stool samples for metabolome analysis.Metabolite identification aims to analyze the metabolome composition of biological samples, investigate the regulation of metabolic pathways, and explore biochemical mechanisms. | The testing time points are set as follows: before exercise, immediately after exercise, and at 24 and 48 hours post-exercise. |
| Before exercise |
| Rate of Perceived Exertion(RPE) | Assess perceived exercise intensity. | The testing time points are set as follows: before exercise, at the 5-kilometer mark during exercise, and immediately after exercise. |
| Heart rate | Assess changes in heart rate. | The testing time points are set as follows: before exercise, at the 5-kilometer mark during exercise, and immediately after exercise. |