Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Class |
|---|---|
| Alliance for Potato Research and Education | OTHER |
Not provided
Not provided
Not provided
Not provided
The objective of this study is to investigate the importance of leavy vegetable preloading on the postprandial glycaemic and insulinemic response in human subjects when consumed a specific amount of digestible carbohydrate from Russet Burbank potatoes source.
The proposed research is part of strategies to manipulate postprandial glycemia and generate weight-control-related benefits, such as promoting satiation. Literature has shown pre-loading caloric nutrients can change postprandial glycemia, but the mechanism is unclear. The investigators hypothesize that a small number of nutrients released in the small intestine can initiate physiological changes and develop feedback control to delay gastric emptying, leading to the delay in digestion of glycemic carbohydrates and the consequent moderate glycemic responses. The investigators propose to use potatoes as the testing glycemic carbohydrates, and the pre-load foods are green vegetables. The investigators will be testing the optimal preload time to achieve the highest control of glycemic response as well as the strongest effect in satiety induction. In addition, green vegetables will be consumed with or without a preload fat enhancer (canola oil) to investigate the mechanism behind significant preload compositions. Healthy adults will be instructed to eat the leafy vegetables first (with or without canola oil), followed by the potato foods (i.e., mashed potatoes). The measurement is the gastric emptying time, blood sugar concentration, insulin, and an appetite-related gut hormone GLP-1. In order to monitor gastric emptying using a non-invasive approach, a popular breath test used in children and a hydrogen breath test will be used to present the change of gastric emptying and the level of gut fermentation.
Results will demonstrate the mechanism of the impact of pre-loading nutrients on the digestion of glycemic carbohydrates. The goal is to eliminate the negative influence of glycemic carbohydrate consumption. Instead, to generate health benefits from dietary carbohydrates.
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Control (All 24 participants) | No Intervention | Subjects consume 300 g of Russet Burbank mashed Potatoes | |
| Co-ingestion (All 24 participants) | Experimental | Subjects co-ingest both (300 g mashed potatoes) and (200 g baby round spinach supplemented with canola oil) in the same time. |
|
| 0 min preload (meal sequence) (10 participants) | Experimental | Subjects consume 200 g baby round spinach supplemented with canola oil first, then directly consume 300 g of mashed potatoes. |
|
| 5 min preload (10 participants) | Experimental | Subjects consume 200 g baby round spinach supplemented with canola oil first, wait 5 minutes, then consume 300 g of mashed potatoes. |
|
| 10 min preload (10 participants) | Experimental | Subjects consume 200 g baby round spinach supplemented with canola oil first, wait 10 minutes, then consume 300 g of mashed potatoes. |
|
| 15 min preload (10 participants) |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Co-ingestion | Other | Subjects co-ingest both (300 g mashed potatoes) and (200 g baby round spinach supplemented with canola oil) in the same time. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Postprandial blood glucose | Blood samples are taken at baseline, 15, 30, 45, 60, 90, 120, 150 and 180 minutes. Glucose levels are tested in the serum using cobas c311 analyzer. | up to 3 hour (180 minutes) after test meal consumption |
| Postprandial blood Insulin | Blood samples are taken at baseline, 15, 30, 45, 60, 90, 120, 150 and 180 minutes. Insulin levels are tested in the serum using cobas e411 Immunoanalyzer. | up to 3 hour (180 minutes) after test meal consumption |
| Postprandial Glucagon Like Peptide -1 Total (GLP-1) | Blood samples are taken at baseline, 15, 30, 45, 60, 90, 120, 150 and 180 minutes. GLP-1 levels are tested in the plasma using GLP-1 Total ELISA kit (EZGLP1T-36K) | up to 3 hour (180 minutes) after test meal consumption |
Not provided
Not provided
Inclusion Criteria:
Exclusion Criteria:
Males
Not provided
Not provided
Not provided
| Name | Affiliation | Role |
|---|---|---|
| Amy Lin, PhD | Senior Principal Scientist I at Singapore Institute of Food and Biotechnology Innovation | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Clinical Nutrition Research Centre (CNRC) | Singapore | 117599 | Singapore |
According to the approved IRB# 2021-095, all IPD are owned by Singapore Institute of Food and Biotechnology Innovation (SIFBI)
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Experimental |
Subjects consume 200 g baby round spinach supplemented with canola oil first, wait 15 minutes, then consume 300 g of mashed potatoes. |
|
| 20 min preload (10 participants) | Experimental | Subjects consume 200 g baby round spinach supplemented with canola oil first, wait 20 minutes, then consume 300 g of mashed potatoes. |
|
| Spinach only /10 min preload (All 24 participants) | Experimental | Subjects consume 200 g baby round spinach supplemented with canola oil first, wait 10 minutes, then consume 300 g of mashed potatoes. |
|
| (Spinach + Canola oil) 10 min preload (All 24 participants) | Experimental | Subjects consume 200 g baby round spinach WITHOUT canola oil first, wait 10 minutes, then consume 300 g of mashed potatoes. |
|
| Canola oil only / 10 min preload (All 24 participants) | Experimental | Subjects consume 20 g of canola oil first, wait 10 minutes, then consume 300 g of mashed potatoes. |
|
| 0 min preload | Other | Subjects consume 200 g baby round spinach supplemented with canola oil first, then directly consume 300 g of mashed potatoes. |
|
| 5 min preload | Other | Subjects consume 200 g baby round spinach supplemented with canola oil first, wait 5 minutes, then consume 300 g of mashed potatoes. |
|
| 10 min preload | Other | Subjects consume 200 g baby round spinach supplemented with canola oil first, wait 10 minutes, then consume 300 g of mashed potatoes. |
|
| 15 min preload | Other | Subjects consume 200 g baby round spinach supplemented with canola oil first, wait 15 minutes, then consume 300 g of mashed potatoes. |
|
| 20 min preload | Other | Subjects consume 200 g baby round spinach supplemented with canola oil first, wait 20 minutes, then consume 300 g of mashed potatoes. |
|
| Spinach only / 10 min preload | Other | Subjects consume 200 g baby round spinach WITHOUT canola oil first, wait 10 minutes, then consume 300 g of mashed potatoes. |
|
| Canola oil only / 10 min preload | Other | Subjects consume 20 g of canola oil first, wait 10 minutes, then consume 300 g of mashed potatoes. |
|
| ID | Term |
|---|---|
| D006943 | Hyperglycemia |
| D006946 | Hyperinsulinism |
| ID | Term |
|---|---|
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |
Not provided
Not provided