Not provided
Not provided
| ID | Type | Description | Link |
|---|---|---|---|
| 2023/15008-9 | Other Grant/Funding Number | Fundação de Amparo à Pesquisa do Estado de São Paulo |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Class |
|---|---|
| Fundação de Amparo à Pesquisa do Estado de São Paulo | OTHER_GOV |
Not provided
Not provided
Not provided
Not provided
Aging is a multifactorial process marked by several epigenetic and molecular changes, such as telomere shortening, DNA exposure to damage, mitochondrial dysfunction, accumulation of senescent cells, and oxidative stress. Such changes lead to the degeneration of cells and molecules, which affects tissues, organs, and systems over time, reducing the human body's ability to resist damage and favoring the development of chronic diseases such as Type 2 Diabetes Mellitus (T2DM). Taurine, a semi-essential amino acid, appears to be related to oxidative homeostasis, glucose control, and inflammation. Therefore, knowing the plasma concentrations of this amino acid in different populations is necessary to obtain a better understanding of the role of taurine in the pathophysiology of obesity and T2DM in older population, in addition to generating hypotheses regarding interventions capable of attenuating oxidative stress and inflammation, which are important hallmarks of aging.
The aim of this study is to investigate whether health status (women with obesity, T2DM and no comorbidities) promotes changes in plasma taurine concentration and to correlate this concentration with biochemical markers (e.g. blood glucose, total cholesterol, insulin, glycated hemoglobin, triglycerides and HDL cholesterol). As well as to evaluate and compare the physical capacities of the different groups. Methods: Forty older women will participate in the cross-sectional study and will be allocated into four distinct groups, and will be classified as follows: older women with obesity and T2DM, older women with obesity and no T2DM, older women without obesity and with T2DM; eutrophic older women, with 10 older women in each group. The following will be performed: body composition assessment by total and regional body scanning using iDXA, anthropometry, tests to assess physical performance, peripheral blood collection for analysis of biochemical markers and the Physical Activity Level will be assessed using the Modified Baecke Questionnaire for Olders. For statistical analysis, One-way ANOVA will be used to verify the differences and compare the four groups. Expected results: It is expected that older women with obesity and T2DM will have lower plasma taurine concentration and also lower performance in physical tests, compared to the other groups.
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| GIObDM - Older women with obesity and type 2 diabetes mellitus | Obesity will be assessed using the Body Mass Index (BMI), with older women being considered obese if they have a BMI above 30 km/m² (OPAS, 2002) |
| |
| GIOb - Older women with obesity and without type 2 diabetes mellitus | Obesity will be assessed using the Body Mass Index (BMI), with elderly women being considered obese if they have a BMI above 30 km/m² (OPAS, 2002) |
| |
| GIDM - Older women without obesity and with type 2 diabetes mellitus | The groups with DM2 will include elderly women who have already been medically diagnosed with DM2 , have been using a hypoglycemic/antidiabetic medication for at least 6 months and have fasting blood glucose ≥ 126mg/dL and/or HbA1c ≥ 6.5% in a blood test in the last 6 months. |
| |
| GIEut - Eutrophic older women | For the group of eutrophic older women, they must have a BMI ≥ 23 and ≤ 28 kg/m² (OPAS, 2002). The medications used by all participants will be surveyed, as well as their dosages and daily consumption. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| None (Observational study) | Other | None (Observational study) |
|
| Measure | Description | Time Frame |
|---|---|---|
| Plasma taurine concentration | Collected in EDTA tubes, were centrifuged and the plasma taurine concentration was evaluated using HPLC (high performance liquid chromatography). | 1 day at baseline |
| Measure | Description | Time Frame |
|---|---|---|
| Lipid Profile | Serum levels of HDL, total cholesterol and triglycerides will be assayed (mg/dL) | 1 day at baseline |
| Glycemic Profile | Serum levels of estimated average blood glucose (mg/dL) |
Not provided
Inclusion Criteria:
Have not exercised regularly for at least 3 months;
Exclusion Criteria:
Not provided
The sample will consist of 40 elderly women who will be allocated into four distinct groups with 10 elderly women in each group.
Not provided
| Name | Affiliation | Role |
|---|---|---|
| Adelino Sanchez Ramos da Silva, PhD | University of Sao Paulo | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Escola de Educação Física e Esporte de Ribeirão Preto | Ribeirão Preto | São Paulo | 14040900 | Brazil |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| ID | Term |
|---|---|
| D009765 | Obesity |
| D003924 | Diabetes Mellitus, Type 2 |
| D003920 | Diabetes Mellitus |
| ID | Term |
|---|---|
| D050177 | Overweight |
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
Not provided
Not provided
| ID | Term |
|---|---|
| D019370 | Observation |
| ID | Term |
|---|---|
| D008722 | Methods |
| D008919 | Investigative Techniques |
Not provided
Not provided
Not provided
Not provided
Not provided
Blood samples will be collected in the morning, after 12 hours of fasting, by experienced nurses. 5 ml of blood will be collected for analysis of biochemical markers (after centrifugation and separation of plasma/serum, they will be stored in a freezer at -80°C for analysis).
The evaluation of biochemical markers will involve the quantification of insulin sensitivity, glycemia, HbA1c, total cholesterol, insulin, triglycerides and HDL cholesterol. The kits Liquiform Total Cholesterol, HDL Cholesterol, Liquiform Triglycerides and Liquiform Glucose from Labtest diagnóstica® will be used, using an enzymatic system and absorbance spectrophotometer.
| 1 day at baseline |
| Glycated hemoglobin | Serum levels glycated hemoglobin (%) | 1 day at baseline |
| Macronutrients intake | The participants dietary intake will be assessed using a three-day dietary recall. The records must be completed by the participants on two weekdays and one weekend day. The professional Dietbox software will be used to calculate the caloric intake of macronutrients | 1 day at baseline |
| Microminerals intake | The participants dietary intake will be assessed using a food registry of 3 days. The records must be completed by the participants on two weekdays and one weekend day. The professional Dietbox software will be used to calculate the caloric intake of microminerals | 1 day at baseline |
| Level of consumption of ultra-processed foods | This study will evaluate the level of consumption of ultra-processed foods. The NOVA classification will be used for this purpose. | 1 day at baseline |
| Upper limb strength endurance test: forearm flexion | Upper limb strength endurance test: forearm flexion for 30 seconds; To assess the strength endurance of the upper limbs, the forearm flexion test will be applied. The number of repetitions in 30 seconds will be counted. | 1 day at baseline |
| Sit-to-Stand-Test | Sit-to-Stand-Test (30 seconds). This test will be used to quantify the lower limb strength of older women, and is recommended in the literature for this population. The number of repetitions in 30 seconds will be counted. | 1 day at baseline |
| Lower limb muscle strength on an adjustable isokinetic dynamometer. | The angular velocities that will be used in the test will be 60º/s. A series of ten repetitions will be performed for the dominant limb for familiarization, followed by a 3-minute rest. | 1 day at baseline |
| Body fat percentage (%) | Using the total body scan from the Dual-energy X-ray absorptiometry (DXA), body fat percentage will be assessed | 1 day at baseline |
| Six-minute walk test | This test aims to assess aerobic capacity. The 6-minute walk test uses a 45.7 m total rectangular path: 18.28 m long by 4.57 m wide. The participant will be instructed to walk as fast as possible (without running) during the 6 minutes, as many times as they can in the rectangle. At the end of the time, the evaluator will record the total distance covered by the participant in meters. | 1 day at baseline |
| Measurement of manual grip strength | The older woman's forearm should be positioned in half pronation, with the wrist in a neutral position. The hand should be positioned on the dynamometer, which will be held by the evaluator. Three attempts will be made with the dominant hand, with 3 seconds of grip and one-minute intervals between them. Of the three attempts, the one with the highest peak force (the largest measurement) will be recorded, in kilograms of force. | 1 day at baseline |
| D001835 |
| Body Weight |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D004700 | Endocrine System Diseases |