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In this study, we investigated the effects of physical activity level and sleep quality level on total antioxidant level (TAS), total oxidant level (TOS), Malondialdehyde (MDA) and Superoxide Dismutase (SOD) which are markers of oxidative stress, irisin, fibronectin type III-protein 5 (FNDC5).
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| T2DM-BMI0 |
| ||
| T2DM-BMI1 |
| ||
| T2DM-BMI2 |
| ||
| T2DM-BMI3 |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Biomarkers / blood | Other | Analysis of routine biochemical parameters |
|
| Measure | Description | Time Frame |
|---|---|---|
| biomarkers derived from blood sera | blood glucose, total cholesterol, triglycerides, LDL and HDL analyzes | 10 Minutes |
| ELISA method of oxidative stress markers | All reagents of the ELISA kits of the oxidative stress markers TAS, TOS, MDA and SOD will be brought to room temperature. Standards will be prepared according to the protocol and added to a 96-well plate with the samples. Buffer solution will be added to each well and incubated for 90 min at room temperature. After each step, necessary washes will be performed and finally chromogenic agents will be added and read spectrophotometrically. Although the timing and additions may vary according to the parameter, in general the procedure is as indicated. Each sample will be run 2 times for both TAS and TOS | 10 minutes |
| Analysis of exercise-related markers by ELISA method | 10 minutes | |
| International Physical Activity Questionnaire | 5 minutes | |
| Pittsburgh Sleep Quality Index | 5 minutes |
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Inclusion Criteria:
Exclusion Criteria:
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Patients applying to the endocrinology department at the medical faculty hospital
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Selcuk University | Konya | Selcuklu | 42130 | Turkey (Türkiye) |
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| ID | Term |
|---|---|
| D003924 | Diabetes Mellitus, Type 2 |
| ID | Term |
|---|---|
| D003920 | Diabetes Mellitus |
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |
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| ID | Term |
|---|---|
| D015415 | Biomarkers |
| D001800 | Blood Specimen Collection |
| D011795 | Surveys and Questionnaires |
| ID | Term |
|---|---|
| D001685 | Biological Factors |
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
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| Questionnaire | Other | Physical activity level will be assessed using the International Physical Activity Questionnaire Short Form (IPQI) and sleep quality will be assessed using the Pittsburgh Sleep Quality Index (PSQI). The data to be obtained from blood will be obtained by selecting blood samples that will be sent to biological waste. |
|
| D004700 | Endocrine System Diseases |
| D003933 | Diagnosis |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |
| D003625 | Data Collection |
| D004812 | Epidemiologic Methods |
| D017531 | Health Care Evaluation Mechanisms |
| D011787 | Quality of Health Care |
| D017530 | Health Care Quality, Access, and Evaluation |
| D011634 | Public Health |
| D004778 | Environment and Public Health |