The fresh pulpy kiwi will be bought 200 gm of kiwi fruits were cleaned, cut into round shapes and blended with 15 mL of distilled water in a blender to obtain about 50 mL of kiwi concentrate.

This concentrate will be filtered and then transferred into a cooling centrifuge to be processed at 2000 rpm for about 20 minutes at a temperature of 40°C.

The extract thus collected will be stored at 4°C till further use.

A 2,350 ppm ozone concentration at a 615 cc per minute flow rate will be supplied by a well-known ozone producing machine.

The ppm of ozone supplied will be verified via an ozone detection device, and the ozone flow rate will be verified by a flow meter directly before the start of the experiment.

Ozone gas will be distributed to the tooth surfaces through special trays to assure a perfect seal to prevent any ozone leakage and to be safely employed to humans

The fresh pulpy kiwi will be bought 200 gm of kiwi fruits were cleaned, cut into round shapes and blended with 15 mL of distilled water in a blender to obtain about 50 mL of kiwi concentrate.

This concentrate will be filtered and then transferred into a cooling centrifuge to be processed at 2000 rpm for about 20 minutes at a temperature of 40°C.

The extract thus collected will be stored at 4°C till further use. A 2,350 ppm ozone concentration at a 615 cc per minute flow rate will be supplied by a well-known ozone producing machine.

The ppm of ozone supplied will be verified via an ozone detection device, and the ozone flow rate will be verified by a flow meter directly before the start of the experiment.

Ozone gas will be distributed to the tooth surfaces through special trays to assure a perfect seal to prevent any ozone leakage and to be safely employed to humans.

Bleaching

all participants will be requested to return after 24 hours to permit rehydration of tooth surfaces before shade assessment Tooth Shades will then record using colorimeter. The color change (ΔE) will be calculated using the formula: ΔE= {(ΔL) 2+ (Δa) 2+ (Δb)

all participants will be requested to return after one month for tooth shades recording using colorimeter. The color change (ΔE) will be calculated using the formula: ΔE= {(ΔL) 2+ (Δa) 2+ (Δb) at one month

all participants will be requested to return after one month for tooth shades recording using colorimeter. The color change (ΔE) will be calculated using the formula: ΔE= {(ΔL) 2+ (Δa) 2+ (Δb) at three months

all participants will be requested to return after one month for tooth shades recording using colorimeter. The color change (ΔE) will be calculated using the formula: ΔE= {(ΔL) 2+ (Δa) 2+ (Δb) at six months