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The purpose of this study; In periodontally healthy individuals, high sensitive c-reactive protein (hs-CRP) levels of non-smokers, smokers of less than 10 cigarettes per day and smokers of more than 10 cigarettes per day were measured at 15 rpm-10 min, 10 centrifuge values, which are frequently used in saliva studies. To determine the levels in saliva samples as a result of rpm-10 min and 10 rpm 6 min centrifugations and to determine the values at which a cytokine of this molecular weight should be centrifuged and to examine the possible correlation between these values and the clinical parameters of the amount of smoking. Materials and Methods: saliva samples were collected from 90 systemically healthy individuals who were non-smokers (N, n=30), light smokers <10 cigarettes per day (L, n=30) and heavy smokers >10 cigarettes per day (H, n=20). samples were taken. Full-mouth clinical periodontal measurements, including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI), and plaque index (PI), were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine hs-CRP levels in biological samples.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Non-Smokers | Experimental | Full-mouth clinical periodontal measurements recorded and saliva obtained. |
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| Light Smokers | Experimental | Full-mouth clinical periodontal measurements recorded and saliva obtained. |
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| Heavy Smokers | Experimental | Full-mouth clinical periodontal measurements recorded and saliva obtained. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| saliva obtaining | Diagnostic Test | The patient was asked to sit upright and tilt his/her head forward to collect saliva samples. İn this way, unstimulated saliva was allowed to accumulate in the floor of the mouth. The accumulated saliva was collected in a sterile container. It was then transferred to a propylene tube. The tubes were centrifuged and the clear part at the top of the tube was taken with a sterile syringe and transferred to a different propylene tube with 0.5 ml in each tube. Tubes were stored at -80ºC until the day of analysis. |
| Measure | Description | Time Frame |
|---|---|---|
| The total amount of hs-CRP in saliva | 24 hours after taking the clinical measurements at the first visit |
| Measure | Description | Time Frame |
|---|---|---|
| Determining the ideal centrifugal value range of hs-CRP saliva level | Within 1 week after receiving the biochemical analysis results |
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Inclusion Criteria:
Systemically healthy
Periodontally clinical healthy
At least 20 permanent teeth in the mouth
No medication for continuous use
Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
Not in pregnancy or lactation period.
Not received periodontal treatment in the last 6 months
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Ece Özer | Contact | +90 5306958625 | ozerrece@gmail.com | |
| Mehmet Sağlam | Contact |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Izmir Katip Çelebi University Department of Periodontology | Recruiting | Izmir | Çiğli | 35640 | Turkey (Türkiye) |
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| ID | Term |
|---|---|
| D012907 | Smoking |
| ID | Term |
|---|---|
| D001519 | Behavior |
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According to full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL),bleeding on probing (BOP), gingival index (GI) and plaque index (PI) samples of saliva were obtained from 90 systemically healthy non-smoker individuals with heavy smokers (H, n=30), light smokers(L, n=30) and non-smokers (N, n=30).
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