The aim of this observational study is to find out whether there is a level difference in salivary mitofusin-1 and mitofusin-2 levels due to changes in periodontal status. The main question it aims to answer is:

• Do salivary mitofusin-1 and mitofusin-2 levels of periodontitis patients differ from healthy patients?
• Could salivary mitofusin-1 and mitofusin-2 levels be biomarkers for periodontal disease?

Biochemical analysis will be performed on saliva samples taken at once from gingivitis or periodontitis patients and healthy individuals who apply to the periodontology clinic, at the initial stage of the treatment. There is no ongoing monitoring or second sampling.

Inclusion Criteria:

• Being between the ages of 18 and 65.
• Willing to participate in the study and provide a saliva sample.
• Having a healthy periodontal condition, gingivitis, or periodontitis (Stage 3-4, Grade C).
• Having fasted from any food or drink (except water) for the past 2 hours prior to providing the saliva sample.
• Having refrained from brushing teeth or using mouthwash in the past 2 hours prior to providing the saliva sample.
• Not having any systemic diseases.
• Not currently taking any medications regularly.

Exclusion Criteria:

• Having used antibiotics within the last 6 months.
• Having received periodontal treatment within the last 6 months.
• Having fewer than 20 teeth (excluding third molars).
• Having any systemic diseases.
• Not smoking or having quit at least 5 years ago.

Patients with gingivitis and periodontitis presenting to the periodontology clinic, as well as healthy individuals