Effects Of Vitamin D On Bone, Muscle, And Adipose Tissue In Obese Subjects

Effects Of Vitamin D On Bone, Muscle, And Adipose Tissue In Obese Subjects: A Randomized, Double-Blind, Placebo-Controlled Study

Obesity has emerged as a risk factor in the onset of bone, muscle and adipose tissue impairments that are further aggravated by vitamin D deficiency. A link of an active bone-muscle-adipose axis is represented by Wnt pathway. This study will test the hypothesis that vitamin D improves bone, muscle, and adipose tissue health through a positive modulation of Wnt pathway. It will be carried out a double-blind, placebo-controlled study of cholecalciferol supplementation in vitamin D-deficient obese adults. Specific aims will be: 1) to test the direct effect of vitamin D on Wnt signaling in bone, muscle, and adipose tissue; 2) to evaluate muscle mass and strength; 3) to assess changes in vitamin D status across different administration strategy (weekly, fortnightly, monthly).

This study will provide not only insight of new mechanisms involved in the pathophysiology of obesity-related musculoskeletal impairments but also evidence for new treatment recommendations for vitamin D deficiency in obesity.

Inclusion Criteria:

• Ambulatory willing and able to provide informed consent;
• post-menopausal women (55-75 y.o.) and age-matched men;
• BMI 30 >= kg/m2;
• serum 25OHD < 20 ng/ml
• hip replacement surgery due to osteoarthritis according to orthopedic clinical decision

Exclusion Criteria:

• eGFR <40 ml/min/1.72 m2 by EPI formula (21);
• hypercalcemia (>10.5 mg/dL);
• osteoporosis (hip or vertebral t-score >-2.5);
• conditions affecting bone
• vitamin D and/or calcium metabolism (chronic liver disease, renal failure, malabsorption, hypercortisolism);
• medications altering bone metabolism (e.g. denosumab, bisphosphonates, teriparatide, glucocorticoids, aromatase inhibitors, estrogen);
• enrollment in an interventional clinical trial in the previous 3 months.