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This prospective multicenter study aims to enroll GBM patients who will undergo repeated assessments (preoperatively and 3 months post-surgery) to detect circulating tumor cells and analyze the transcriptomic profiles of EVs in their blood. The prognostic and monitoring significance of these biomarkers to disease course (assessing treatment efficacy, resistance incidence, tumor progression) will be evaluated.
Concurrently, proteomic profiles typical of GBM will be analyzed in blood and ocular secretion samples from GBM patients, patients with low-grade gliomas, and patients without brain tumors to identify and validate novel protein biomarkers suitable for disease monitoring. Additionally, this study proposes an innovative approach to monitor GBM patients by investigating the presence of GBM-specific nucleic acid fragments in urine.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Glioblastoma multiforme | Patients with GBM |
| |
| Low-grade glioma | Patients with low-grade gliomas. |
| |
| Control group | Patients without cancer or systemic inflammation |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Diagnostic test | Diagnostic Test | No intervention among the arms. The diagnostic, prognostic, predictive and follow-up value of tested biomolecules will be analyzed. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Proteomic profiles of proximal liquids in brain tumor patients | The protein profiles will be measured in ocular secretion and plasma samples of patients wiht GBM, low-grade glioma and control cohort. Candidate proteins will be correleted with clinical parameters. The number of patients with identified prognostic biomarkers will be reported. | 2020-2023 |
| Circulating tumor cells (CTCs) for non-invasive monitoring of GBM patients. | The number of CTCs and their molecular characteristics will be measured in peripheral blod of GBM patients. The CTCs counts will be correleted with the clinical parameters. The number of patients with the presence or absence of CTCs will be reported. | 2020-2030 |
| Extracellular vesicles (EVs) for non-invasive monitoring of GBM patients. | The RNA profiles of EVs will be measured in plasma samples of patients wiht GBM. Candidate RNAs will be correleted with clinical parameters. The number of patients with identified prognostic RNAs will be reported. | 2020-2030 |
| Urine as a source of GBM-specific nucleic acids fragments for disease monitoring. | The GBM-specific nucleic acids fragments will be measured in urine of GBM patients. Candidate nucleic acids will be correleted with clinical parameters. The number of patients with identified prognostic biomolecules will be reported. | 2025-2030 |
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Inclusion Criteria: Adult patients with brain tumors -
Exclusion Criteria: No other cancer; no inflammatory disease
-
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Inhabitants of Olomouc region
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Josef Srovnal, MD,PhD | Contact | +420585632137 | josef.srovnal@upol.cz | |
| Ondrej Kalita, MD,PhD | Contact | +420588443568 | ondrej.kalita@fnol.cz |
| Name | Affiliation | Role |
|---|---|---|
| Marian Hajduch, MD,PhD | Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Department of Neurosurgery, University Hospital Olomouc | Recruiting | Olomouc | 77900 | Czechia |
Anonymous proteomic profiles of plasma and ocular secretion and transcriptomic data from extracelular vesicles will be shared.
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| ID | Term |
|---|---|
| D001932 | Brain Neoplasms |
| ID | Term |
|---|---|
| D016543 | Central Nervous System Neoplasms |
| D009423 | Nervous System Neoplasms |
| D009371 | Neoplasms by Site |
| D009369 | Neoplasms |
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| ID | Term |
|---|---|
| D003955 | Diagnostic Tests, Routine |
| ID | Term |
|---|---|
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
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| D001927 |
| Brain Diseases |
| D002493 | Central Nervous System Diseases |
| D009422 | Nervous System Diseases |