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This study focuses on detecting the possible beneficial effect of Lactoferrin (Lf) administration on the kidney and the heart of glycerol-induced rhabdomyolysis (RM) in rats and its relation to ferroptosis and AMPK/Nrf2/ HO-1 signalling pathway. The main questions it aims to answer are:
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Control | Sham Comparator | Normal saline will be administered once to rats intramuscularly (10 mL/kg). |
|
| RM | Active Comparator | A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and normal saline (4ml/kg) will be administered by gavage. |
|
| Lf-RM | Active Comparator | A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. before RM induction |
|
| RM-Lf | Active Comparator | A single intramuscular injection of GLY 50% (10 mL/kg) after 24-h water deprivation and Lf (1000 mg/kg in normal saline, volume 4 mL/kg) will be administered by gavage 30 min. after RM induction. |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Lactoferrin | Drug | an iron-binding protein |
| |
| Measure | Description | Time Frame |
|---|---|---|
| Nrf2 expression | Nrf2 expression in heart and kidney tissues | 2 months |
| AMPK levels | AMPK levels in kidney and heart tissue homogenate | 2 months |
| HO-1 levels | HO-1 levels in kidney and heart tissue homogenate | 2 months |
| Measure | Description | Time Frame |
|---|---|---|
| iron levels | Iron levels in heart and kidney tissue homogenate | 2 months |
| MDA levels | MDA levels in heart and kidney tissue homogenate |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Faculty of Medicine | Aswān | Aswan Governorate | 81528 | Egypt |
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| Label | URL |
|---|---|
| Related Info | View source |
| Related Info | View source |
| Related Info |
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| ID | Term |
|---|---|
| D012206 | Rhabdomyolysis |
| ID | Term |
|---|---|
| D009135 | Muscular Diseases |
| D009140 | Musculoskeletal Diseases |
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| ID | Term |
|---|---|
| D007781 | Lactoferrin |
| D005990 | Glycerol |
| ID | Term |
|---|---|
| D012697 | Serine Endopeptidases |
| D010450 | Endopeptidases |
| D010447 | Peptide Hydrolases |
| D006867 | Hydrolases |
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| iron |
| Diagnostic Test |
Iron in kidney and heart tissues |
|
| MDA, TAC, and GSH | Diagnostic Test | Oxidative stress levels in kidney and heart tissues |
|
|
| Nrf2 | Diagnostic Test | Nrf2 expression in kidney and heart tissues |
|
|
| AMPK | Diagnostic Test | AMPK levels in heart and kidney tissues |
|
|
| HO-1 | Diagnostic Test | HO-1 levels in kidney and heart tissues |
|
|
| glycerin | Drug | GLY intramuscular injection for rhabdomyolysis induction |
|
|
| 2 months |
| TAC levels | TAC levels in heart and kidney tissue homogenate | 2 months |
| GSH levels | GSH levels in heart and kidney tissue homogenate | 2 months |
| Related Info | View source |
| Related Info | View source |
| D004798 |
| Enzymes |
| D045762 | Enzymes and Coenzymes |
| D057057 | Serine Proteases |
| D006023 | Glycoproteins |
| D006001 | Glycoconjugates |
| D002241 | Carbohydrates |
| D061250 | Transferrins |
| D033862 | Iron-Binding Proteins |
| D002352 | Carrier Proteins |
| D011506 | Proteins |
| D000602 | Amino Acids, Peptides, and Proteins |
| D007782 | Lactoglobulins |
| D000067816 | Whey Proteins |
| D008894 | Milk Proteins |
| D000080224 | Animal Proteins, Dietary |
| D004044 | Dietary Proteins |
| D005916 | Globulins |
| D008667 | Metalloproteins |
| D000073999 | Triose Sugar Alcohols |
| D013402 | Sugar Alcohols |
| D000438 | Alcohols |
| D009930 | Organic Chemicals |