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Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions from Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections that will be obtained under complete aseptic precautions.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| group1 | Pseudomonas Aeruginosa Isolates |
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| Measure | Description | Time Frame |
|---|---|---|
| Isolation and identification of pseudomonas aeruginosa causing different nosocomial infections. | Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions | 6 months |
| Identification of antibiotic susceptibility pattern of isolated strains of pseudomonas | Antibiotic susceptibility testing of the isolates will be performed using disc diffusion method according to clinical laboratory standards institute (CLSI) guidelines | 6 months |
| Detection of biofilm forming isolates | Detection of biofilm forming isolates by tissue culture plate method. | 6 months |
| Detection of virulence and antibiotic resistance genes of pseudomonas aeruginosa. | Detection of virulence and antibiotic resistance genes by conventional Polymerase Chain Reaction | 6 months |
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Inclusion Criteria:
Exclusion Criteria:
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Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections will be obtained under complete aseptic precautions.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Martina Gamil Fayez, Demonstrator at Microbiology | Contact | 01272602453 | martina.gameel@med.sohag.edu.eg |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Faculty of medicine Sohag university hospital | Recruiting | Sohag | Egypt |
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| Type | Date | Date Unknown |
|---|---|---|
| Release | Jan 28, 2026 | |
| Reset | Feb 13, 2026 | |
| Release | Apr 7, 2026 | |
| Reset | Apr 28, 2026 | |
| Release | May 1, 2026 | |
| Reset | May 26, 2026 |
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| Release Date | Unrelease Date | Unrelease Date Unknown | Reset Date | MCP Release Number |
|---|---|---|---|---|
| Jan 28, 2026 | Feb 13, 2026 | |||
| Apr 7, 2026 |
| ID | Term |
|---|---|
| D011552 | Pseudomonas Infections |
| ID | Term |
|---|---|
| D016905 | Gram-Negative Bacterial Infections |
| D001424 | Bacterial Infections |
| D001423 | Bacterial Infections and Mycoses |
| D007239 | Infections |
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| Apr 28, 2026 |
| May 1, 2026 | May 26, 2026 |