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L-Arginine is an amino acid capable of stimulating the production of nitric oxide (NO) metabolites. NO is responsible for several important activities of the body, such as: helping to regulate vasodilation, blood flow, mitochondrial respiration, platelet function and increasing growth hormone levels, but the effects on the recovery of cardiorespiratory parameters after exercise testing maximum need to be clarified.
The protocols will be carried out at the Stress Physiology Laboratory of the Faculty of Science and Technology, UNESP-Presidente Prudente. The execution time of the protocols will be between 5:00 pm and 9:00 pm, to standardize the circadian influence on the evaluated parameters, the environmental climate will be controlled with a temperature between 23ºC and 24 ºC and humidity between 60% and 70%. Volunteers will be instructed not to drink alcoholic or caffeine-based drinks for 12 hours before all protocols, to consume a light meal and 500 ml of water two hours before and to avoid vigorous physical exertion the day before. The experimental procedure will be divided into two stages, all carried out on a treadmill, with a minimum interval of 48 hours between them, in order to allow adequate recovery. Before the start of the first stage of the protocols, anthropometric measurements will be taken, body weight using a digital scale and height using a stadiometer.
Before the beginning of each stage, a Polar RS800CX heart rate monitor will be placed to record the heart rate (HR) and its variability, remaining until the end of each protocol. Afterwards, he will sit for 60 minutes of initial rest and HR, Systolic Blood Pressure (SBP), Diastolic Blood Pressure (DBP), oxygen saturation (SpO2) and Heart Rate Variability (HRV) will be measured. After these measurements, volunteers will go to the second phase:
Placebo protocol (PP): consisting of the prior consumption of 3g of placebo (starch) and subsequently resting for 60 minutes, sitting, then a submaximal exercise test on an ergometric treadmill or;
Arginine (PA) experimental protocol: in this stage, volunteers will perform activities similar to the PC, but they previously consumed 3g of L-arginine 60 minutes before the submaximal test and then;
Perform physical exercise on a treadmill with a 1% incline for the first 3 minutes to warm up at a speed of 5km/h, followed by load increments of 1km/h every 2 minutes until reaching 80% of the maximum HR estimated by age (208-( 0.7*age) with the same inclination. At the end of the activity, you will remain lying down in a calm environment where HR, SBP, DBP, Spo2 and HRV will be monitored for another 20 minutes in the 1st, 3rd, 5th, 7th, 10th and 20th. minute.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Sun | Experimental | We are blind and don't know which group is which. |
|
| Moon | Placebo Comparator | We are blind and don't know which group is which. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| L-arginine | Dietary Supplement | Capsules of 3 grams of L-arginine per Dose |
| |
| Measure | Description | Time Frame |
|---|---|---|
| Autonomic Nervous System | Heart Rate Variability | 10 minutes previous to exercise and 20 minutes after exercise. |
| Measure | Description | Time Frame |
|---|---|---|
| Blood Pressure | 10 minutes previous to exercise and 20 minutes after exercise. | |
| Heart Rate | 10 minutes previous to exercise and 20 minutes after exercise. | |
| Oxygen Saturation |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| UNESP | Recruiting | Presidente Prudente | São Paulo | 19060-900 | Brazil |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 40389021 | Derived | Porto AA, Gonzaga LA, Gomes RL, Candeloro BM, Raimundo RD, Vanderlei LCM, Valenti VE. Acute effects of l-arginine intake on heart rate variability after a submaximal exercise test in healthy men: randomized clinical trial. Nitric Oxide. 2025 Aug;157:68-73. doi: 10.1016/j.niox.2025.05.003. Epub 2025 May 17. |
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| ID | Term |
|---|---|
| D001120 | Arginine |
| ID | Term |
|---|---|
| D024361 | Amino Acids, Basic |
| D000596 | Amino Acids |
| D000602 | Amino Acids, Peptides, and Proteins |
| D000599 | Amino Acids, Diamino |
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| Placebo |
| Dietary Supplement |
Capsules of 3 grams of maize Starch per Dose |
|
| 10 minutes previous to exercise and 20 minutes after exercise. |
| D000601 | Amino Acids, Essential |