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moraxella catarrhalis is responsible for respiratory tract infection in children and adults with streptococcus pneumonia and haemophilus influenza.Moraxella catarrhalis is gram negative diplococci, non-motile and non spore bearing bacteria. Until, 1995 it was considered as a non pathogenic respiratory tract flora.This bacteria is an important pathogen and a common cause of both upper and lower respiratory tract infections, pneumonia, sinusitis and conjunctivitis in infants, children and in elderly patients. In adults, M. catarrhalis also causes chronic obstructive pulmonary disease (COPD) and pneumonia. However, it is associated with a number of respiratory infections affecting both children and adults, including laryngitis, bronchitis and pneumonia .
This institution is polymicrobial community with other pathogens such as Streptococcus pneumoniae and Haemophilus influenza (Sethi and Murphy, 2008). The pathogenicity of M. catarrhalis is mediated by several virulence genes, including the ubiquitous surface protein genes A1 (uspA1) and A2 (uspA2), which encode virulence factors that promote colonization and successful host infections (Bernhard S et al.,2012).Bacterium was first isolated in 1896, it was considered to be a harmless commensal of the upper respiratory tract for a long period of time. The bacterium rapidly colonizes the nasopharynx soon after birth asymptomatically (Blakeway et al., 2017).
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| Measure | Description | Time Frame |
|---|---|---|
| isolation of moraxella catarrhalis | isolation of organism on muller hinton agar | 3 years |
| Measure | Description | Time Frame |
|---|---|---|
| detection of virulence genes of moraxella catarrhalis | detection of virulence genes by PCR | 3 years |
| detetion of antibiotic resistance | detetion of antibiotic resistance by agar disc diffusion |
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Inclusion Criteria:
Exclusion Criteria:
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children with respiratory tract infection
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 19038881 | Background | Sethi S, Murphy TF. Infection in the pathogenesis and course of chronic obstructive pulmonary disease. N Engl J Med. 2008 Nov 27;359(22):2355-65. doi: 10.1056/NEJMra0800353. No abstract available. | |
| 28893369 | Background | Blakeway LV, Tan A, Peak IRA, Seib KL. Virulence determinants of Moraxella catarrhalis: distribution and considerations for vaccine development. Microbiology (Reading). 2017 Oct;163(10):1371-1384. doi: 10.1099/mic.0.000523. Epub 2017 Sep 12. |
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ethical consideration
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| ID | Term |
|---|---|
| D012141 | Respiratory Tract Infections |
| ID | Term |
|---|---|
| D007239 | Infections |
| D012140 | Respiratory Tract Diseases |
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sputum samples for DNA extraction and PCR
| 3years |
| 23136074 | Background | Bernhard S, Spaniol V, Aebi C. Molecular pathogenesis of infections caused by Moraxella catarrhalis in children. Swiss Med Wkly. 2012 Oct 29;142:w13694. doi: 10.4414/smw.2012.13694. eCollection 2012. |