Hepatocellular Carcinoma Liver Organoids

Hepatocellular Carcinoma Organoids to Explore Tumor Microenvironment and Test Treatment Efficacy - Hepatocellular Carcinoma Liver Organoids (HELIO)

The goal of this interventional study is to create hepatocellular carcinoma organoids from liver bioptic samples of individuals with hepatocellular carcinoma. The main questions it aims to answer are:

• the feasibility of hepatocellular carcinoma organoids integrated with host gut microbiota and peripheral blood mononuclear cells
• the molecular pattern of the organoid tumor microenvironment
• the in vitro therapeutic response of hepatocellular carcinoma organoids

Inclusion Criteria:

• Ability to express informed consent
• Patients ≥ 18 years of age
• Radiological diagnosis of hepatocellular carcinoma (HCC)

Exclusion Criteria:

• Patients <18 years of age
• Contraindications for liver biopsy: ascites, platelet count <50.000, International Normalized Ratio (INR) >1.7
• Active viral infection
• Refusal to give informed consent for the study