Multiplex PCR for Severe Respiratory Infections During the COVID-19 Pandemic

Relevance of the Multiplex PCR Approach for Severe Respiratory Infections of Patients Hospitalized - Including an Intensive Care Unit, During the COVID-19 Pandemic

The hypothesis was that a retrospective investigation of the molecular virological tests in a University hospital could be informative, with the aim to identify non-COVID-19 respiratory viruses during the circulation of SARS-CoV-2, according to systematic population data for public health knowledge.

The hypothesis was that a retrospective investigation of the molecular virological tests in a University hospital could be informative, with the aim to identify non-COVID-19 respiratory viruses during the circulation of SARS-CoV-2, according to systematic population data for public health knowledge:

• the main goal was to perform a retrospective investigation of the molecular virological tests in a University hospital, in order to identify non-COVID-19 respiratory viruses from the beginning of 2021 to mid-2022 during the circulation of SARS-CoV-2, for public health knowledge;
• the secondary aim was to retrospectively analyze, for a small subgroup of patients from an intensive care unit, the files of patients with positive molecular virological tests for non-COVID-19 respiratory viruses from the beginning of 2021 to mid-2022, reporting the following standard-of-care data: (i) the identification of the viruses, (ii) bioclinical features, (iii) medical impact of virological results in real life.

Inclusion Criteria:

• For adult patients, or minors 'via' parents, able to give consent - 18 intensive care patients

Exclusion Criteria:

• Non-inclusion in case of refusal