In conclusion, the study shows that the hospital-based group had a significantly higher screening uptake than the community-based group, reflecting that information from ophthalmologists and registered ophthalmic nurses is more effective and well-received than that from village health volunteers. The study suggests that increasing education and support for village health volunteers could enhance screening uptake. Factors influencing uptake include the age of FDRs, place of residence, education, presenting VA of probands, and treatment of probands. Utilizing this study's findings, spreading knowledge and training health officials at the district and sub-district levels could increase understanding of glaucoma and improve communication with the general public and at-risk groups, potentially increasing the response rate. Additionally, implementing a national free screening glaucoma program for at-risk populations, both in and outside hospitals, and organizing mobile screening units at the district level could be an effective prevention strategy against permanent vision loss from glaucoma.

Inclusion Criteria:

• All patients with POAG who had a treatment history in the ophthalmology outpatient department of Prapokklao Hospital, Chanthaburi Province, were identified from the Electronic Medical Record (EMR) database with the 10th revision of the International Statistical Classification of Diseases and Related Health Problems (ICD-10) " Primary open angle glaucoma H40.1" between 1 january 2022 to 31 october 2022. A total of 1304 patients were included in this study.

Exclusion Criteria:

• Excluding those with closed-angle glaucoma and secondary glaucoma due to other conditions such as accidents, post-surgical complications, uveitis, or congenital.

These probands were then divided into two non-randomized groups: The first group, hospital-based, included all probands receiving treatment in the ophthalmology outpatient department from 1 dec 2022 to 28 feb 2023. These patients received information about glaucoma from ophthalmologists and registered ophthalmic nurses, along with a brochure.

These patients were asked to invite FDRs-parents, siblings, and offspring aged 40 years and above - to participate screening.

The second group, community-based, involved randomly selecting districts in Chanthaburi In this group, village health volunteers trained in glaucoma knowledge by ophthalmologists distributed brochures and educated patients and FDRs, similar to the hospital-based group. These FDRs were then invited to participate in the glaucoma screening program one month later, with ophthalmologists at health stations near homes in Muang district and Laem Sing Hospital between 1 mar 2023 to 31 may 2023.