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| ID | Type | Description | Link |
|---|---|---|---|
| TSA-2020-1045 | Other Grant/Funding Number | Scientific Research Projects Coordination Unit |
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The objective of the study will be to investigate the effect of low-level diode laser therapy (LLLT) and steroid on facial nerve injury. Thirty five male Wistar rat will randomly be divided into five groups: healthy control (HCG); damage control (DC); laser (LG); steroid (SG); and combined laser and steroid group (LSG). Right facial nerve electromyography (EMG) data will record after facial nerve damage done and before sacrification at 4th week.The results will be evaluated histopathologically, electrophysiologically and biochemically.
The animals will be randomly divided into two control groups, a healthy control group and a damaged control group, and three experimental groups; 7 rats were assigned to each group as follows;
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Healthy Control Group (HCG) | Placebo Comparator | Seven rats will be included randomly in this group. No surgical/medical approach will be applied. |
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| Damaged Control Group (DCG): | Active Comparator | Facial nerve injury will be created on the right facial nerve. No surgical/medical approach will be applied. |
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| Laser Group (LG) | Experimental | Facial nerve injury will be created on the right facial nerve. LLLT (Low-Intensity et al.) willl be applied 3 times a week for 4 weeks postoperatively, at 830 nm with an optical power output of 30 mW, an energy density of 4 J/cm2, an irradiation area of 0.116 cm2 and an exposure time of 16 s per spot. |
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| Steroid group (SG) | Experimental | Facial nerve injury will be created on the right facial nerve. Intraperitoneal methylprednisolone (1mg/kg/day) will be administered every day for 1 week postoperatively. |
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| Laser+Steroid Group (LSG): | Experimental | Facial nerve injury will be created on the right facial nerve. Laser and steroid treatment will be applied in combination. |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Methyprednisolon and low level laser theraphy | Drug | All rats will be anesthetized with ketamine hydrochloride at a dose of 50 mg/kg (i.p) (Ketalar ®, Eczacıbaşı Parke-Davis, İstanbul, Türkiye) and Xylazine HCl (Alfazyne ®, Alfasan International B.V. Woerden, Hollanda) at a dose of 10 mg/kg (intraperitoneal). A right postauricular incision will made under general anesthesia, and an external trunk of the facial nerve will be found. In the DCG and other experimental groups, the facial nerve was dissected, and it will be clamped with an average force of 150 g for about 1 minute. Afterward, the surgical area will be sutured in all rats in the DCG, L, S, and LSG groups. At the end of the 4 weeks of study, tissue samples will be taken from the facial nerve trunk in the control and experimental groups with a second surgical procedure preserved in formalin solution and sent for histopathological and biochemical examination. |
| Measure | Description | Time Frame |
|---|---|---|
| Electromyographic Analysis | Measuring the amplitude and latency of the action potential occurring in the nerve with electromyography(On facial nerve) | 1 month |
| Measure | Description | Time Frame |
|---|---|---|
| Biochemical Analysis | Determination of MDA and GSH levels in blood samples taken | 1 month |
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Inclusion Criteria:
-Thirty-five male Sprague-Dawley rats weighing 200-300 g will be included the study
Exclusion Criteria:
-Rats that are older than 6 months and are not healthy and have impaired facial motor functions will be excluded from the study.
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| Name | Affiliation | Role |
|---|---|---|
| zeynep gümrükçü, Associate Professor | RTEU | Study Director |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| RTEU Faculty of Dentistry | Rize | Turkey (Türkiye) |
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| ID | Term |
|---|---|
| D020220 | Facial Nerve Injuries |
| ID | Term |
|---|---|
| D005155 | Facial Nerve Diseases |
| D009059 | Mouth Diseases |
| D009057 | Stomatognathic Diseases |
| D020209 | Cranial Nerve Injuries |
| D003389 | Cranial Nerve Diseases |
| D009422 | Nervous System Diseases |
| D006259 | Craniocerebral Trauma |
| D020196 | Trauma, Nervous System |
| D014947 | Wounds and Injuries |
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