Transcriptional and Proteomic Analysis of Acute Kidney Injury

Transcriptional and Proteomic Analysis of Acute Kidney Injury

Acute kidney injury is associated with worsened outcome for critically ill patients. Sepsis-associated and non-septic cardiothoracic surgery associated AKI have been reported, nonetheless, precise pathomechanistic differences as well as detectability of transcriptional and proteomic changes in correlation with imaging and plasma markers are unclear.

Acute kidney injury is a common and detrimental finding in critically ill/sepsis patients as well as non-septic patients post cardiothoracic surgery. In many cases renal replacement therapy is required and an acute kidney injury is linked to increased morbidity and mortality in intensive care patients. Using routinely obtained clinical samples as well as imaging data, this observational trial investigates the transcriptomic and proteomic determinants detectable in septic and non-septic AKI patient subsets and their correlation with outcome and AKI diagnostic parameters.

• Inclusion criteria:

  • study consent;
  • KDIGO>=2;
  • vasoplegia and/or sepsis or elective nephrectomy.

• Exclusion criteria:

  • Age<18 years;
  • pregnancy/nursing;
  • preexisting CKD with eGFR<60m/min;
  • dialysis; former ogran transplantation;
  • HIV; hematologic malignancies;
  • immunsuppression;
  • 4h post biopsy non-pausable effective anticoagulation;
  • pre-existing coagulopathy