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The T cell characteristics of 12 patients treated with CD20 CAR-T (LY007 cell injection) were analyzed to study their relationship with CAR-T anti-tumor activity, tumor killing, and in vivo proliferation, and to explore the mechanisms: before single harvesting, before bridging, before pretreatment, D0, D7, D14, D21, D28, and at the time of follow-up evaluation (plus time points if taking BTKi inhibitors: Peripheral blood specimens were collected before BTKi and 48h after BTKi discontinuation, and peripheral blood cells from patients before and after treatment were analyzed by mass spectrometry flow and single-cell sequencing.
Tumor tissue specimens were collected from patients at different time points (before pretreatment, during CAR-T expansion, and at PD) and subjected to single-cell sequencing.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Patients receive LY007 CD20 CAR-T | |||
| Patients receive commerial CD19 CAR-T in China |
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| Measure | Description | Time Frame |
|---|---|---|
| Expression levels of genes (known to be possible driver genes in lymphoma) analyzed from tumor tissues collected before treatment and at the point of progression if available for DNA sequencing after quality control. | Tumor tissues collected before treatment and at the point of progression if available for DNA sequencing after quality control. On the one hand, the difference of tumor mutations will be analyzed in pre-treatment biopsy samples of patients grouped by efficacy; on the other hand, the changes of tumor mutations will be analyzed between pre-treatment and post-treatment biopsy samples of patients. | 2023.10-2025.12 |
| Expression levels of genes (known to be possible driver genes in lymphoma) analyzed from tumor tissues collected before treatment and at the point of progression if available for RNA sequencing after quality control. | Tumor tissues collected before treatment and at the point of progression if available for bulky RNA sequencing, single-cell RNA sequencing and spatial transcriptome sequencing after quality control. On the one hand, the difference of characteristics of tumor microenvironment will be analyzed in pre-treatment biopsy samples of patients grouped by efficacy; on the other hand, the changes of characteristics of tumor microenvironment will be analyzed between pre-treatment and post-treatment biopsy samples of patients. | 2023.10-2025.12 |
| Level of M1 macrophage in peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry. | Peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry to analyze the changes of immune celltypes and immune checkpoints. | 2023.10-2025.12 |
| Level of M2 macrophage in peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry. | Peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry to analyze the changes of immune celltypes and immune checkpoints. |
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Inclusion Criteria:
Exclusion Criteria:
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Inpatients and outpatients from Shanghai Ruijin Hospital
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Zixun Yan, Doctor | Contact | +862164370045 | 610707 | yzx12119@rjh.com.cn |
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| ID | Term |
|---|---|
| D008228 | Lymphoma, Non-Hodgkin |
| ID | Term |
|---|---|
| D008223 | Lymphoma |
| D009370 | Neoplasms by Histologic Type |
| D009369 | Neoplasms |
| D008232 | Lymphoproliferative Disorders |
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| 2023.10-2025.12 |
| Level of OX-40 in peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry. | Peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry to analyze the changes of immune celltypes and immune checkpoints. | 2023.10-2025.12 |
| Level of PD-1 in peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry. | Peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry to analyze the changes of immune celltypes and immune checkpoints. | 2023.10-2025.12 |
| Level of TIM-3 in peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry. | Peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry to analyze the changes of immune celltypes and immune checkpoints. | 2023.10-2025.12 |
| Level of LAG-3 in peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry. | Peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry to analyze the changes of immune celltypes and immune checkpoints. | 2023.10-2025.12 |
| Level of lymphocyte subpopulations in peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry. | Peripheral blood collected before each cycle and at the point of progression if available for Mass Cytometry to analyze the changes of immune celltypes and immune checkpoints. | 2023.10-2025.12 |
| D008206 |
| Lymphatic Diseases |
| D006425 | Hemic and Lymphatic Diseases |
| D007160 | Immunoproliferative Disorders |
| D007154 | Immune System Diseases |