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The goal of this observational study is to learn about clinical application of pathogenic metagenomic next-generation sequencing to optimize the diagnosis of infection in decompensated cirrhotic patients. The main questions it aims to answer are:
Metagenomic next-generation sequencing (mNGS) is emerging as an important culture-independent technique that can detect nearly all known pathogens simultaneously from a clinical sample.Sequencing of microbial cell-free DNA (cfDNA) has recently been shown to enable diagnosis of several infection. Relevant studies on the clinical application of mNGS in cirrhosis patients are rare. The primary aim of this study was to comprehensively evaluate the fragments of genomic DNA from circulating microorganisms in acutely decompensated cirrhosis patients by sequencing the microbial cfDNA and relate this to clinical outcomes. The secondary aim was to validate the potential role of CMV reactivation, a known NHV with available antiviral medicines, in determining the prognosis of decompensated cirrhosis patients.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| patients received mNGS test | After enrolment, patients will receive mNGS test. |
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| Measure | Description | Time Frame |
|---|---|---|
| Positive rate of mNGS test in AD patients | at enrolment |
| Measure | Description | Time Frame |
|---|---|---|
| 90-day transplantation-free mortality | From enrollment to 90 days | |
| Incidence of acute kidney injury (AKI) | AKI is defined as a change in SCr of ≥ 0.3 mg/dl (26.5 μmol/L) in ≤ 48 h, or a 50% increase in SCr from a baseline that is known or presumed to have occurred in the past 7 days. |
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Inclusion Criteria:
Exclusion Criteria:
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Patient with diagnosis of cirrhosis hospitalized for acute decompensation
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Chen Jinjun | Contact | +86 020 62787310 | chjj@smu.edu.cn |
| Name | Affiliation | Role |
|---|---|---|
| Chen Jinjun | Nanfang Hospital, Southern Medical University | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Nanfang Hospital | Recruiting | Guangzhou | Guangdong | China |
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| ID | Term |
|---|---|
| D008103 | Liver Cirrhosis |
| ID | Term |
|---|---|
| D008107 | Liver Diseases |
| D004066 | Digestive System Diseases |
| D005355 | Fibrosis |
| D010335 | Pathologic Processes |
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Whole blood, urine, feces
| From enrollment to 90 days |
| Proportion of hospital readmissions due to infections | From enrollment to 90 days |
| Proportion of progression to SIRS or sepsis | From enrollment to 90 days |
| Consistency with blood culture results | Concordance of mNGS With Other traditional Testing on Pathogens | at enrollment |
| Incidence of CMV reactivation | Cytomegalovirus DNA was quantified in stored plasma samples using real-time PCR (polymerase chain reaction) assay. DNA extraction was performed on 200 µL of plasma using a QIAamp DNA blood kit (Qiagen, German). Then, 25 µL of Tris (10 mM, pH 8.0) was used to elute the DNA, and 10 µL of the DNA was used for each PCR reaction. The minimum detection level was 102 copies/ml of plasma and values over this lower detection limit were considered to be CMV reactivation positive. | From enrollment to 90 days |
| Rate of progression to acute-on-chronic liver failure (ACLF) | ACLF was defined according to the European Association for the Study of Liver-Chronic Liver Failure (EASL-CLIF) criteria. ACLF grade-1 includes three subgroups: 1) patients with single kidney failure; 2) patients with single failure of the liver, coagulation, circulation or respiration, who had serum creatinine ranging from 1.5 to 1.9 mg/dl and/or mild-to-moderate hepatic encephalopathy; and 3) patients with single cerebral failure who had serum creatinine ranging from 1.5 and 1.9 mg/dl. ACLF grade-2: patients with two organs failure. ACLF grade-3: patients with three organ failures or more. ACLF development: patients with absence of ACLF on admission and progression to ACLF within 28 days. The severity of liver disease was evaluated by the model of end-stage liver disease (MELD) score, Child-Pugh score and CLIF-AD score (in those without ACLF). | From enrollment to 90 days |
| D013568 |
| Pathological Conditions, Signs and Symptoms |