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In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes, and identify the microRNA expression in the spent media of the blastocysts cultures.
In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes. By this way, we assume to predict which follicle is able to give the best blastocyst. After the blastocysts are obtained from the different sized follicles, blastocyst cultures are going to be studied for the up and down-regulations of microRNA, besides the correlation of embryo quality with miRNA expression in the spent media.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Women referring to infertility clinic for IVF treatment | Women who were referred to infertility clinic with complaint of not having baby go through IVF treatment, and having oocyte pick-up procedure with different sizes of ovarian follicles |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Oocyte pick-up | Procedure | With the needle, transvaginally USG-guided oocyte pick-up procedure is going to be performed |
|
| Measure | Description | Time Frame |
|---|---|---|
| Oocyte grading | Morphologic Oocyte grading will be analyzed right after the procedure due to different follicle sizes | after each hyper stimulation process of the patients, when the follicle sizes are large enough to pick up which is roughly 10-15 days after the hyper stimulation has started |
| Day-3 Embryo Grading | Morphologic Day-3 embryo grading will be analyzed after the procedure due to different follicle sizes | Three days after the intracytoplasminc sperm injection(ICSI) procedure |
| Day-5 Embryo Grading | Day-5 embryo grading(Gardner classification) will be analyzed after the procedure due to different follicle sizes | 5 days after the intracytoplasminc sperm injection(ICSI) procedure |
| beta hCG results of the transferred embryos | Clinical pregnancy outcomes | ten days after the embryo transfer |
| Measure | Description | Time Frame |
|---|---|---|
| micro RNA expression in culture medium | In the culture medium of blastocysts are going to be analyzed for microRNA levels that are expressed | Culture mediums of day 5 embryos will be collected to be studied, after the last embryo is obtained and all will be studied at the end of the recruitment |
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Inclusion Criteria:
Exclusion Criteria:
Patients must be all female, since the study is based on embryos obtained from different ovarian follicle sizes
Patients who are going to be included to the study will be the patients of infertility clinic
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| NAZLI ALBAYRAK, MD | Contact | 00905054111626 | albayraknalzi@gmail.com | |
| TURGUT AYDIN, MD | Contact | 00905323722713 | turgut.aydin@acibadem.com |
| Name | Affiliation | Role |
|---|---|---|
| NAZLI ALBAYRAK, MD | Acıbadem University School of Medicine | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Acibadem University Atakent Hospital | Recruiting | Istanbul | 34303 | Turkey (Türkiye) |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 35367061 | Background | Shapiro BS, Rasouli MA, Verma K, Raman A, Garner FC, Aguirre M, Kaye L, Bedient C. The effect of ovarian follicle size on oocyte and embryology outcomes. Fertil Steril. 2022 Jun;117(6):1170-1176. doi: 10.1016/j.fertnstert.2022.02.017. Epub 2022 Mar 30. | |
| 32389425 | Background | Mohr-Sasson A, Orvieto R, Blumenfeld S, Axelrod M, Mor-Hadar D, Grin L, Aizer A, Haas J. The association between follicle size and oocyte development as a function of final follicular maturation triggering. Reprod Biomed Online. 2020 Jun;40(6):887-893. doi: 10.1016/j.rbmo.2020.02.005. Epub 2020 Feb 20. |
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IPD is not going to be shared with other researchers. All data is going to be known only by one researcher.
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| 31786607 | Background | Costermans NGJ, Soede NM, van Tricht F, Blokland M, Kemp B, Keijer J, Teerds KJ. Follicular fluid steroid profile in sows: relationship to follicle size and oocyte qualitydagger. Biol Reprod. 2020 Mar 13;102(3):740-749. doi: 10.1093/biolre/ioz217. |
| 18249377 | Background | Rosen MP, Shen S, Dobson AT, Rinaudo PF, McCulloch CE, Cedars MI. A quantitative assessment of follicle size on oocyte developmental competence. Fertil Steril. 2008 Sep;90(3):684-90. doi: 10.1016/j.fertnstert.2007.02.011. Epub 2008 Feb 4. |
| 30282571 | Background | Annes K, Muller DB, Vilela JAP, Valente RS, Caetano DP, Cibin FWS, Milazzotto MP, Mesquita FS, Belaz KRA, Eberlin MN, Sudano MJ. Influence of follicle size on bovine oocyte lipid composition, follicular metabolic and stress markers, embryo development and blastocyst lipid content. Reprod Fertil Dev. 2019 Mar;31(3):462-472. doi: 10.1071/RD18109. |
| 32966987 | Background | Orvieto R, Mohr-Sasson A, Blumenfeld S, Nahum R, Aizer A, Haas J. Does a Large (>24 mm) Follicle Yield a Competent Oocyte/Embryo? Gynecol Obstet Invest. 2020;85(5):416-419. doi: 10.1159/000510876. Epub 2020 Sep 23. |
| 34155958 | Background | Kasapoglu I, Orhan A, Aslan K, Sen E, Kaya A, Avci B, Uncu G. Are all antral follicles the same? Size of antral follicles as a key predictor for response to controlled ovarian stimulation. J Obstet Gynaecol. 2022 Apr;42(3):461-466. doi: 10.1080/01443615.2021.1910640. Epub 2021 Jun 22. |
| 9683350 | Background | Saith RR, Srinivasan A, Michie D, Sargent IL. Relationships between the developmental potential of human in-vitro fertilization embryos and features describing the embryo, oocyte and follicle. Hum Reprod Update. 1998 Mar-Apr;4(2):121-34. doi: 10.1093/humupd/4.2.121. |
| 7957985 | Background | Wittmaack FM, Kreger DO, Blasco L, Tureck RW, Mastroianni L Jr, Lessey BA. Effect of follicular size on oocyte retrieval, fertilization, cleavage, and embryo quality in in vitro fertilization cycles: a 6-year data collection. Fertil Steril. 1994 Dec;62(6):1205-10. doi: 10.1016/s0015-0282(16)57186-6. |
| 32485714 | Background | Orvieto R, Mohr-Sasson A, Aizer A, Nahum R, Blumenfeld S, Kirshenbaum M, Haas J. Do Follicles of Obese Patients Yield Competent Oocytes/Embryos? Gynecol Obstet Invest. 2020;85(3):290-294. doi: 10.1159/000508226. Epub 2020 Jun 2. |