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This research aims to elucidate an underlying mechanism of maternal obesity induced pregnancy and longterm health complications for mothers and their offspring.
With the increasing global prevalence of obesity, pregnancy problems related to maternal obesity are increasingly occurring. Microbial gut symbiosis plays an important role in health, with dysbiosis being associated with diseases such as obesity. Of interest are pregnancy, dietary patterns and pre- or probiotics that affect the composition of the gut microbiome. The microbiome itself can influence many physiological processes, such as immune responses (production of microbial products) and the nutrient-dependent one-carbon metabolism. It is hypothesized that gut dysbiosis, due to maternal obesity, during pregnancy can be considered an endogenous chronic stressor causing impaired immune response and carbon metabolism. Both processes result in excessive oxidative stress, detrimental to cell replication, differentiation and epigenetic programming of maternal and infant tissues. Together, these biological disturbances contribute to placental and vascular dysfunction, leading to an increased risk of preeclampsia or gestational diabetes mellitus. Vertical (during pregnancy) and horizontal (during delivery) transmission of gut dysbiosis from mother to newborn and epigenetic placental and foetal changes may ultimately lead to macrosomia and obesity in children. Therefore, the differences between the gut and vaginal microbiome, maternal and fetal immune responses and one-carbon metabolism in obese versus normal-weight pregnant women will be analysed.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| 110 women | 60 women with a BMI between 18,5-25 kg/m2, of which 10 preconceptional 60 women with a BMI > 30 kg/m2, of which 10 preconceptional |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Blood withdrawal | Other | venous punction with blood withdrawal Vaginal and rectal swab, done by patient itself |
|
| Measure | Description | Time Frame |
|---|---|---|
| Gut and vaginal microbiota | Composition of gut and vaginal microbiota derived by swab sampling, bacteriome profiles will be assessed by 16S ribosomal ribonucleic acid (16SrRNA) gene amplification sequencing (V6-V8). Sequences will be assigned to operational taxonomic units (OTUs). | Preconceptional (up to 1 year before pregnancy) |
| Gut and vaginal microbiota | Composition of gut and vaginal microbiota derived by swab sampling, bacteriome profiles will be assessed by 16SrRNA gene amplification sequencing (V6-V8). Sequences will be assigned to OTUs. | First trimester (between 7-12 weeks gestational age) |
| Gut and vaginal microbiota | Composition of gut and vaginal microbiota derived by swab sampling, bacteriome profiles will be assessed by 16SrRNA gene amplification sequencing (V6-V8). Sequences will be assigned to OTUs. | Second trimester (between 22-25 weeks gestational age) |
| Gut and vaginal microbiota | Composition of gut and vaginal microbiota derived by swab sampling, bacteriome profiles will be assessed by 16SrRNA gene amplification sequencing (V6-V8). Sequences will be assigned to OTUs. | Third trimester (between 30-32 weeks gestational age) |
| Gut and vaginal microbiota | Composition of gut and vaginal microbiota derived by swab sampling, bacteriome profiles will be assessed by 16SrRNA gene amplification sequencing (V6-V8). Sequences will be assigned to OTUs. | Antepartum (during delivery) |
| Gut and vaginal microbiota | Composition of gut and vaginal microbiota derived by swab sampling, bacteriome profiles will be assessed by 16SrRNA gene amplification sequencing (V6-V8). Sequences will be assigned to OTUs. |
| Measure | Description | Time Frame |
|---|---|---|
| Gut virome | Composition of gut virome, obtained by a rectal swab | Preconceptional (up to 1 year before pregnancy) |
| Gut virome | Composition of gut virome, obtained by a rectal swab |
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Inclusion Criteria:
Exclusion Criteria:
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This pilot study is embedded in the Rotterdam Periconceptional Cohort study (Predict study). The Predict study population includes preconceptional or pregnant women > 18 years and < 45 years old visiting the Erasmus MC and that are willing to participate. We will select the participants, that meet our undermentioned inclusion criteria, for our study from this cohort: 50 women with a BMI > 30 kg/m2 (cases) and 50 women with a BMI ranging 18-25 kg/m2 (controls) and their neonates.
We will longitudinally sample first/2nd/3rd/postpartum, 100 women, in addition we will include 10 preconceptional women with BMI > 30 kg/m2 and 10 preconceptional women with BMI 18-25 kg/m2, these participants do not necessarily have to conceive a pregnancy in order to remain in the study (these women are part of the preconceptional Predict population).
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Nicole Schenkelaars, MD | Contact | +31627530793 | n.schenkelaars@erasmusmc.nl |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Erasmus MC | Recruiting | Rotterdam | South Holland | 3015GD | Netherlands |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 34097026 | Background | Rousian M, Schoenmakers S, Eggink AJ, Gootjes DV, Koning AHJ, Koster MPH, Mulders AGMGJ, Baart EB, Reiss IKM, Laven JSE, Steegers EAP, Steegers-Theunissen RPM. Cohort Profile Update: the Rotterdam Periconceptional Cohort and embryonic and fetal measurements using 3D ultrasound and virtual reality techniques. Int J Epidemiol. 2021 Nov 10;50(5):1426-1427l. doi: 10.1093/ije/dyab030. No abstract available. | |
| 23784909 |
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| Type | Includes Protocol | Includes SAP | Includes ICF | Document Label | Document Date | Document Uploaded Date | Document File Name |
|---|---|---|---|---|---|---|---|
| Prot_SAP | Yes | Yes | No | Study Protocol and Statistical Analysis Plan | Jun 15, 2022 | Feb 2, 2023 | Prot_SAP_000.pdf |
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| ID | Term |
|---|---|
| D000079262 | Pregnancy in Obesity |
| D011248 | Pregnancy Complications |
| ID | Term |
|---|---|
| D005261 | Female Urogenital Diseases and Pregnancy Complications |
| D000091642 | Urogenital Diseases |
| D009765 | Obesity |
| D050177 | Overweight |
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Blood samples Vaginal sample (swab) Rectal sample (swab)
| Postpartum (6-8 weeks post delivery) |
| First trimester (between 7-12 weeks gestational age) |
| Gut virome | Composition of gut virome, obtained by a rectal swab | Second trimester (between 22-24 weeks gestational age) |
| Gut virome | Composition of gut virome, obtained by a rectal swab | Third trimester (between 30-32 weeks gestational age) |
| Gut virome | Composition of gut virome, obtained by a rectal swab | Antepartum (during delivery) |
| Gut virome | Composition of gut virome, obtained by a rectal swab | Postpartum (6-8 weeks post delivery) |
| Maternal immune response | Responses of maternal immune system advanced oxidation protein products (AOPP)) measured in chloramine units per gram of protein (micromol/g) obtained by blood withdrawal and measured in the lab. | Preconceptional (up to 1 year before pregnancy) |
| Maternal immune response | Responses of maternal immune system advanced oxidation protein products (AOPP)) measured in chloramine units per gram of protein (micromol/g) obtained by blood withdrawal and measured in the lab. | First trimester (between 7-12 weeks gestational age) |
| Maternal immune response | Responses of maternal immune system advanced oxidation protein products (AOPP)) measured in chloramine units per gram of protein (micromol/g) obtained by blood withdrawal and measured in the lab. | Second trimester (between 22-24 weeks gestational age) |
| Maternal immune response | Responses of maternal immune system advanced oxidation protein products (AOPP)) measured in chloramine units per gram of protein (micromol/g) obtained by blood withdrawal and measured in the lab. | Third trimester (between 30-32 weeks gestational age) |
| Maternal immune response | Responses of maternal immune system advanced oxidation protein products (AOPP)) measured in chloramine units per gram of protein (micromol/g) obtained by blood withdrawal and measured in the lab. | Antepartum (during delivery) |
| Maternal immune response | Responses of maternal immune system advanced oxidation protein products (AOPP)) measured in chloramine units per gram of protein (micromol/g) obtained by blood withdrawal and measured in the lab. | Postpartum (6-8 weeks post delivery) |
| Maternal immune response | Tumor necrosis factor-alpha (TNF-alpha) measured in picograms per milliliter, obtained by blood withdrawal and measured in the lab. | Preconceptional (up to 1 year before pregnancy) |
| Maternal immune response | Tumor necrosis factor-alpha (TNF-alpha) measured in picograms per milliliter, obtained by blood withdrawal and measured in the lab. | First trimester (between 7-12 weeks gestational age) |
| Maternal immune response | Tumor necrosis factor-alpha (TNF-alpha) measured in picograms per milliliter, obtained by blood withdrawal and measured in the lab. | Second trimester (between 22-24 weeks gestational age) |
| Maternal immune response | Tumor necrosis factor-alpha (TNF-alpha) measured in picograms per milliliter, obtained by blood withdrawal and measured in the lab. | Third trimester (between 30-32 weeks gestational age) |
| Maternal immune response | Tumor necrosis factor-alpha (TNF-alpha) measured in picograms per milliliter, obtained by blood withdrawal and measured in the lab. | Antepartum (during delivery) |
| Maternal immune response | Tumor necrosis factor-alpha (TNF-alpha) measured in picograms per milliliter, obtained by blood withdrawal and measured in the lab. | Postpartum (6-8 weeks post delivery) |
| Maternal immune response | Interleukin-6 (IL-6), measured in picograms per milliliter, obtained by blood withdrawal and measured in the lab. | Preconceptional (up to 1 year before pregnancy) |
| Maternal immune response | Interleukin-6 (IL-6), measured in picograms per milliliter, obtained by blood withdrawal and measured in the lab. | First trimester (between 7-12 weeks gestational age) |
| Maternal immune response | Interleukin-6 (IL-6), measured in picograms per milliliter, obtained by blood withdrawal and measured in the lab. | Second trimester (between 22-24 weeks gestational age) |
| Maternal immune response | Interleukin-6 (IL-6), measured in picograms per milliliter, obtained by blood withdrawal and measured in the lab. | Third trimester (between 30-32 weeks gestational age) |
| Maternal immune response | Interleukin-6 (IL-6), measured in picograms per milliliter, obtained by blood withdrawal and measured in the lab. | Antepartum (during delivery) |
| Maternal immune response | Interleukin-6 (IL-6), measured in picograms per milliliter, obtained by blood withdrawal and measured in the lab. | Postpartum (6-8 weeks post delivery) |
| Maternal immune response | high sensitive C-reactive protein(hsCRP), measured in mg/L, obtained by blood withdrawal and measured in the lab. | Preconceptional (up to 1 year before pregnancy) |
| Maternal immune response | high sensitive C-reactive protein(hsCRP), measured in mg/L, obtained by blood withdrawal and measured in the lab. | First trimester (between 7-12 weeks gestational age) |
| Maternal immune response | high sensitive C-reactive protein(hsCRP), measured in mg/L, obtained by blood withdrawal and measured in the lab. | Second trimester (between 22-24 weeks gestational age) |
| Maternal immune response | high sensitive C-reactive protein(hsCRP), measured in mg/L, obtained by blood withdrawal and measured in the lab. | Third trimester (between 30-32 weeks gestational age) |
| Maternal immune response | high sensitive C-reactive protein(hsCRP), measured in mg/L, obtained by blood withdrawal and measured in the lab. | Antepartum (during delivery) |
| Maternal immune response | high sensitive C-reactive protein(hsCRP), measured in mg/L, obtained by blood withdrawal and measured in the lab. | Postpartum (6-8 weeks post delivery) |
| Maternal metabolic response | Markers of the one-carbon metabolism; folate, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Preconceptional (up to 1 year before pregnancy) |
| Maternal metabolic response | Markers of the one-carbon metabolism; folate, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | First trimester (between 7-12 weeks gestational age) |
| Maternal metabolic response | Markers of the one-carbon metabolism; folate, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Second trimester (between 22-24 weeks gestational age) |
| Maternal metabolic response | Markers of the one-carbon metabolism; folate, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Third trimester (between 30-32 weeks gestational age) |
| Maternal metabolic response | Markers of the one-carbon metabolism; folate, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Antepartum (during delivery) |
| Maternal metabolic response | Markers of the one-carbon metabolism; folate, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Postpartum (6-8 weeks post delivery) |
| Maternal metabolic response | Markers of the one-carbon metabolism; Homocysteine, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Preconceptional (up to 1 year before pregnancy) |
| Maternal metabolic response | Markers of the one-carbon metabolism; Homocysteine, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | First trimester (between 7-12 weeks gestational age) |
| Maternal metabolic response | Markers of the one-carbon metabolism; Homocysteine, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Second trimester (between 22-24 weeks gestational age) |
| Maternal metabolic response | Markers of the one-carbon metabolism; Homocysteine, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Third trimester (between 30-32 weeks gestational age) |
| Maternal metabolic response | Markers of the one-carbon metabolism; Homocysteine, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Antepartum (during delivery) |
| Maternal metabolic response | Markers of the one-carbon metabolism; Homocysteine, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Postpartum (6-8 weeks post delivery) |
| Maternal metabolic response | Markers of the one-carbon metabolism; B-vitamin 12, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Preconceptional (up to 1 year before pregnancy) |
| Maternal metabolic response | Markers of the one-carbon metabolism; B-vitamin 12, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | First trimester (between 7-12 weeks gestational age) |
| Maternal metabolic response | Markers of the one-carbon metabolism; B-vitamin 12, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Second trimester (between 22-24 weeks gestational age) |
| Maternal metabolic response | Markers of the one-carbon metabolism; B-vitamin 12, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Third trimester (between 30-32 weeks gestational age) |
| Maternal metabolic response | Markers of the one-carbon metabolism; B-vitamin 12, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Antepartum (during delivery) |
| Maternal metabolic response | Markers of the one-carbon metabolism; B-vitamin 12, measured in micromol/l, obtained by blood withdrawal and measured in the lab. | Postpartum (6-8 weeks post delivery) |
| Clinical maternal outcome: gestational age | Gestational age (amenorrhea duration) at delivery. | Durante partum |
| Clinical maternal outcome: pre-eclampsia | Pre-eclampsia is defined as the combination of gestational hypertension (systolic blood pressure ≥ 140 mmHg and/or diastolic blood pressure ≥ 90 mmHg (Korotkoff V) occurring after 20 weeks of gestation gestational age, measured twice, in a woman who previously had normal blood pressure) with proteinuria (≥ 300 mg/24 hours). | from 20 weeks of gestation to <8 weeks postpartum |
| Clinical maternal outcome: hypertension | Hypertension is defined as a systolic blood pressure ≥ 140 mmHg and/or diastolic blood pressure ≥ 90 mmHg (Korotkoff V) occurring after 20 weeks of gestation gestational age, measured twice, in a woman who previously had normal blood pressure. | from 20 weeks of gestation to <8 weeks postpartum |
| Clinical maternal outcome: gestational diabetes | Gestational diabetes defined as any form of hyperglycaemia detected during pregnancy, regardless ofwhether this abnormality disappears after pregnancy. Diagnosed through a 75 gr Oral Glucose Tolerance Test (OGTT) with a fasting venous value > 7 mmol/l or above 7.8 mmol/l after 2 hours. | From the first positive pregnancy test to delivery |
| Fetal growth | Fetal growth trajectories, Crown-Rump-Length (CRL) obtained by using ultrasound imaging. | First trimester (Between 7-7+6 days of gestational age) |
| Fetal growth | Fetal growth trajectories, Crown-Rump-Length (CRL) obtained by using ultrasound imaging. | First trimester (Between 9-9+6 days of gestational age) |
| Fetal growth | Fetal growth trajectories, Crown-Rump-Length (CRL) obtained by using ultrasound imaging. | First trimester (Between 11-11+6 days of gestational age) |
| Fetal growth | Fetal growth trajectories defined as Estimated Fetal Weight (EFW) (in grams) based on the measurements (in mm) of the Head circumference (HC), Biparietal diameter (BPD), Abdominal circumference (AC) and Femur length (FL) to be obtained/measured during the ultrasound. | Second trimester (Between AD 22-25 weeks of gestational age) |
| Fetal growth | Fetal growth trajectories defined as Estimated Fetal Weight (EFW) (in grams) based on the measurements (in mm) of the Head circumference (HC), Biparietal diameter (BPD), Abdominal circumference (AC) and Femur length (FL) to be obtained/measured during the ultrasound. | Third trimester (Between 30-33 weeks of gestational age) |
| Histological placental function | Histology of placenta: biopsies are taken within 2 days after delivery, these are snapfrozen in -80 degrees Celsius and assessed according to protocol by pathologist | Postpartum (<2 days postpartum) |
| Placental weight | Placental weight measured (in grams), weighed on the scale. | Postpartum (<2 days postpartum) |
| Background |
| Gaillard R, Durmus B, Hofman A, Mackenbach JP, Steegers EA, Jaddoe VW. Risk factors and outcomes of maternal obesity and excessive weight gain during pregnancy. Obesity (Silver Spring). 2013 May;21(5):1046-55. doi: 10.1002/oby.20088. |
| 26002170 | Background | Mission JF, Marshall NE, Caughey AB. Pregnancy risks associated with obesity. Obstet Gynecol Clin North Am. 2015 Jun;42(2):335-53. doi: 10.1016/j.ogc.2015.01.008. |
| 18331423 | Background | Singh AS, Mulder C, Twisk JW, van Mechelen W, Chinapaw MJ. Tracking of childhood overweight into adulthood: a systematic review of the literature. Obes Rev. 2008 Sep;9(5):474-88. doi: 10.1111/j.1467-789X.2008.00475.x. Epub 2008 Mar 5. |
| 25123127 | Background | Tanvig M. Offspring body size and metabolic profile - effects of lifestyle intervention in obese pregnant women. Dan Med J. 2014 Jul;61(7):B4893. |
| 30006584 | Background | Kuhle S, Muir A, Woolcott CG, Brown MM, McDonald SD, Abdolell M, Dodds L. Maternal pre-pregnancy obesity and health care utilization and costs in the offspring. Int J Obes (Lond). 2019 Apr;43(4):735-743. doi: 10.1038/s41366-018-0149-3. Epub 2018 Jul 13. |
| 24578535 | Background | Morgan KL, Rahman MA, Macey S, Atkinson MD, Hill RA, Khanom A, Paranjothy S, Husain MJ, Brophy ST. Obesity in pregnancy: a retrospective prevalence-based study on health service utilisation and costs on the NHS. BMJ Open. 2014 Feb 27;4(2):e003983. doi: 10.1136/bmjopen-2013-003983. |
| 29968760 | Background | Elderman M, Hugenholtz F, Belzer C, Boekschoten M, de Haan B, de Vos P, Faas M. Changes in intestinal gene expression and microbiota composition during late pregnancy are mouse strain dependent. Sci Rep. 2018 Jul 3;8(1):10001. doi: 10.1038/s41598-018-28292-2. |
| 31523266 | Background | Schoenmakers S, Steegers-Theunissen R, Faas M. The matter of the reproductive microbiome. Obstet Med. 2019 Sep;12(3):107-115. doi: 10.1177/1753495X18775899. Epub 2018 May 17. |
| 19043404 | Background | Turnbaugh PJ, Hamady M, Yatsunenko T, Cantarel BL, Duncan A, Ley RE, Sogin ML, Jones WJ, Roe BA, Affourtit JP, Egholm M, Henrissat B, Heath AC, Knight R, Gordon JI. A core gut microbiome in obese and lean twins. Nature. 2009 Jan 22;457(7228):480-4. doi: 10.1038/nature07540. Epub 2008 Nov 30. |
| 40173397 | Derived | Schenkelaars N, Wekema L, Faas MM, Steegers-Theunissen RPM, Schoenmakers S. Protocol of the PROMOTE study: characterization of the microbiome, the immune response, and one-carbon metabolism in preconceptional and pregnant women with and without obesity (an observational subcohort of the Rotterdam Periconception cohort). PLoS One. 2025 Apr 2;20(4):e0319618. doi: 10.1371/journal.pone.0319618. eCollection 2025. |
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
| D001835 | Body Weight |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |