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Gout is a chronic joint disease associated with deposition of monosodium urate crystals, as a consequence of hyperuricemia. Gout is an intermittent flaring condition and acute gout flares are driven by NLRP3 inflammasome and IL1-beta production.
However pathogenesis of acute gout flares remains poorly known in vivo in human.
The aim of this study is to evaluate NLRP3 inflammasome activation in vivo in patients cells during acute gout flares.
Blood and synovial fluid will be obtained from remainder of patients' samples during an acute gout flares. White blood cells will be isolated and NLRP3 inflammasome activation will be assessed. Then, NLRP3 inflammasome inhibitors will be tested on cells with NLRP3 inflammasome activity.
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| Measure | Description | Time Frame |
|---|---|---|
| NLRP3 inflammasome activation in white blood cells. | White blood cells will be isolated from blood sample and NLRP3 inflammasome activation will be assessed by flow cytometry. | Patient admission at hospital |
| Measure | Description | Time Frame |
|---|---|---|
| NLRP3 inflammasome activation in synovial cells. | Nucleated cells will be isolated from synovial fluid sample and NLRP3 inflammasome activation will be assessed by flow cytometry. | Patient admission at hospital |
| Effects of NLRP3 inflammasome inhibitors on proportion of NLRP3 inflammasome activation in white blood cells/synovial cells |
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Inclusion Criteria:
Exclusion Criteria:
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Study will be proposed to patients meeting study's criteria
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Julien Seiller, MD | Contact | 478 617 757 | +33 | jseiller@chsjsl.fr |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Centre Hospitalier Saint Joseph Saint Luc | Recruiting | Lyon | 69007 | France |
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| ID | Term |
|---|---|
| D015210 | Arthritis, Gouty |
| ID | Term |
|---|---|
| D006073 | Gout |
| D001168 | Arthritis |
| D007592 | Joint Diseases |
| D009140 | Musculoskeletal Diseases |
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Blood and synovial fluid obtained from remainder of samples collected during standard of care required during acute goute flare.
NLRP3 inflammasome inhibitors will be tested on cells with NLRP3 inflammasome activity in blood or synovial fluid. |
| up to 7 days after analysis of NLRP3 activity |
| D000070657 |
| Crystal Arthropathies |
| D012216 | Rheumatic Diseases |
| D011686 | Purine-Pyrimidine Metabolism, Inborn Errors |
| D008661 | Metabolism, Inborn Errors |
| D030342 | Genetic Diseases, Inborn |
| D009358 | Congenital, Hereditary, and Neonatal Diseases and Abnormalities |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |