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| Name | Class |
|---|---|
| German University in Cairo | OTHER |
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Cardiac-enriched micro-RNAs (miRNAs), micro RNA 208b and 133a(MiR-208b, MiR-133a)) corresponds to the health and disorders of the cardiovascular system. An intron of the cardiac myosin heavy chain gene MYH7 encodes miR-208b. It is found on chromosome 14 in humans. Identify new diagnostic biomarkers based on miRNAs, researchers examine the expression of miR-133a and 208b at various time points (04 hours, 08 hours, 12 hours, 24 hours, 48 hours) following the development of the infarct and compared it to the traditional myocardial infarction biomarkers cardiac troponine (cTnl) and Creatine kinase-MB (CK-MB).
The 98% of human genes are non-coding RNA, which is further divided into long non-coding RNA (lncRNA, > 200 bp) and short non-coding RNA (200 bp, including microRNA (miRNA) and circular RNA). More than 2000 human miRNAs have been discovered, and it seems that they regulate around 60% of human genes.Through a number of pathways, miRNAs control the development, re-modelling, and regeneration of the heart as well as endothelial function, vasculo-genesis, and neoangiogenesis. 30% of the genes in the human genome are regulated by about 1000 miRNAs. Endogenous miRNAs are 19-22 nucleotide single-stranded RNA molecules that frequently target the 3' untranslated regions (3'-UTR) of mRNAs to regulate gene expression at the post-transcriptional level in physiological processes, disease development, and conditions like cancer, autoimmune and inflammatory diseases, neuro-degenerative diseases, and cardiovascular diseases. Cardiac-enriched miRNAs, MiR-208 in health and disorders of the cardiovascular system are investigated.
Identify new diagnostic biomarkers based on miRNAs, researchers examine the expression of miR-133a and 208b at various time points (04 hours, 08 hours, 12 hours, 24 hours, 48 hours) following the development of the infarct and compared it to the traditional myocardial infarction biomarkers cTnl and CK-MB.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Test Group | The study group included 10 patients,
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| Control Group | The study group included 10 patients,
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| No intervention | Other | Expression of miR-133a &208b at 4, 8, 12, 24 and 48 hours from the first appearance of myocardial infarction (MI) symptoms |
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| Measure | Description | Time Frame |
|---|---|---|
| Expression of miR-133a | Expression during episodes of myocardial infarction | 04 hours from the onset of the infarct |
| Expression of miR-133a | Expression during episodes of myocardial infarction | 08 hours from the onset of the infarct |
| Expression of miR-133a | Expression during episodes of myocardial infarction | 12 hours from the onset of the infarct |
| Expression of miR-133a | Expression during episodes of myocardial infarction | 24 hours from the onset of the infarct |
| Expression of miR-133a | Expression during episodes of myocardial infarction | 48 hours from the onset of the infarct |
| Expression of miR-208b | Expression during episodes of myocardial infarction | 04 hours from the onset of the infarct |
| Expression of miR-208b | Expression during episodes of myocardial infarction | 08 hours from the onset of the infarct |
| Expression of miR-208b | Expression during episodes of myocardial infarction |
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Exclusion Criteria:
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Patient registering at the Coronary Care Unit at Ain-shams University hospital German University in Cairo, New Cairo City,.Egypt
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| Name | Affiliation | Role |
|---|---|---|
| Sally I Hassanien, Ph.D | Biochemistry Department German University in Cairo, 11835, Cairo, Egypt | Principal Investigator |
| Hafiz A Sattar, M.Phil | University College of Conventional Medicine, The Islamia University of Bahawalpur, Punjab, Pakistan | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Biochemistry Department, Faculty of Pharmacy and Biotechnology, German University in Cairo, Main Entrance Al Tagamoa Al Khames | Cairo | 11835 | Egypt |
De-identified data will be published.
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| ID | Term |
|---|---|
| D009203 | Myocardial Infarction |
| ID | Term |
|---|---|
| D017202 | Myocardial Ischemia |
| D006331 | Heart Diseases |
| D002318 | Cardiovascular Diseases |
| D014652 | Vascular Diseases |
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| 12 hours from the onset of the infarct |
| Expression of miR-208b | Expression during episodes of myocardial infarction | 24 hours from the onset of the infarct |
| Expression of miR-208b | Expression during episodes of myocardial infarction | 48 hours from the onset of the infarct |
| D007238 |
| Infarction |
| D007511 | Ischemia |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D009336 | Necrosis |