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The European Food Safety Authority has approved many applications for UVB light treated foods (e.g. UVB-exposed button mushrooms) in the last years. The UVB light treatment is used to increase the vitamin D content in foods and improve the vitamin D status of subjects. However, UVB irradiation is accompanied by the formation of vitamin D photoisomers such as lumisterol and tachysterol. The current study aims to investigated whether these vitamin D photoisomers can enter the circulation and are metabolised in humans that consume UVB-treated mushrooms.
according to protocol
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| oral intake of UVB-exposed mushrooms | Experimental | Daily intake of 500 g of UVB-exposed button mushrooms over 7 days (provided as mushroom cream soup) and blood sampling (at baseline, 3 h postprandial, 6 h postprandial and at day 8) |
|
| oral intake of non-UVB-exposed mushrooms | Placebo Comparator | Daily intake of 500 g of non-UVB-exposed button mushrooms over 7 days (provided as mushroom cream soup) and blood sampling (at baseline, 3 h postprandial, 6 h postprandial and at day 8) |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| UVB-exposed button mushrooms | Dietary Supplement | Healthy subjects will be randomized into two groups and received either UVB-exposed button mushrooms or non-UVB-exposed button mushrooms for 7 days. Blood samples from each subject will be taken at baseline (before the intake of the mushrooms, 3 h and 6 h thereafter, and at day 8. Three months later, another blood sample was taken. |
| Measure | Description | Time Frame |
|---|---|---|
| Circulating vitamin D photoisomers | Plasma concentrations of vitamin D photoisomers (such as lumisterol, tachysterol and their hydroxy derivates) after the consumption of the UVB-exposed versus non UVB-exposed button mushrooms | at baseline (before the intervention), 3 hours postprandial, 6 hours postprandial, at day 8, changes from baseline at 3 months |
| Measure | Description | Time Frame |
|---|---|---|
| Parameters of vitamin D metabolism | Plasma concentrations of vitamin D metabolites (vitamin D2, vitamin D3, 25-hydroxyvitamin D2, 25-hydroxyvitamin D3, 24,25-dihydroxyvitamin D2, calcitriol, ergosterol) | at baseline (before the intervention), 3 hours postprandial, 6 hours postprandial, at day 8, changes from baseline at 3 months |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Gabriele I Stangl, Prof. Dr. | Contact | +493455522707 | gabriele.stangl@landw.uni-halle.de |
| Name | Affiliation | Role |
|---|---|---|
| Gabriele I Stangl, Prof. Dr. | Martin-Luther-Universität Halle-Wittenberg | Principal Investigator |
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| Parameters of mineral metabolism |
Plasma calcium, phosphate, parathyroid hormone, fibroblast growth factor-23 |
| at baseline (before the intervention), 3 hours postprandial, 6 hours postprandial, at day 8, changes from baseline at 3 months |
| Plasma lipids | Plasma triglycerides, cholesterol | at baseline (before the intervention), 3 hours postprandial, 6 hours postprandial, at day 8, changes from baseline at 3 months |
| Inflammation markers | Plasma CRP, Interleukin-6 | at baseline (before the intervention), 3 hours postprandial, 6 hours postprandial, at day 8, changes from baseline at 3 months |
| mRNA and protein expression in peripheral mononuclear blood cells | Vitamin D receptor target genes, CYP enzymes | at baseline (before the intervention), 3 hours postprandial, 6 hours postprandial, at day 8, changes from baseline at 3 months |