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| Name | Class |
|---|---|
| Belarusian Research Center for Pediatric Oncology, Hematology and Immunology | OTHER |
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The purpose of this study is to develop a highly sensitive method for assessing the functional activity of platelets for use in the differential diagnosis of thrombocytopenia and platelet defects.
The purpose of this study is to develop a highly sensitive method for assessing the functional activity of platelets using flow cytometry and turbidimetric and impedance aggregometry for use in the differential diagnosis of thrombocytopenia and platelet defects
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Control group | Group of healthy adult and pediatric participants, whose blood platelets will be used to evaluate normal ranges of parameters in the flow cytometry PFT. |
| |
| Hemorrhagic syndrome and Acute Leukemia | Group of pediatric participants with any manifestations of hemorrhagic syndrome and/or acute leukemia |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Flow cytometry | Diagnostic Test | Parameters such as FSC, SSC, median fluorescence of anti CD42b, PAC-1, CD41, CD63, CD62P, as well as Annexin V fluorescence will be assessed. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Expression of CD42b by platelets | Assessment of platelet functional activity by the detection of CD42b | baseline |
| Expression of CD41 by platelets | Assessment of platelet functional activity by the detection of CD41 | baseline |
| Expression of PAC-1 by platelets | Assessment of platelet functional activity by the detection of PAC-1 | baseline |
| Expression of CD63 by platelets | Assessment of platelet functional activity by the detection of CD63 | baseline |
| Expression of CD62p by platelets | Assessment of platelet functional activity by the detection of CD62p | baseline |
| Expression of Annexin V by platelets | Assessment of platelet functional activity by the detection of Annexin V | baseline |
| Level of platelet aggregation | Assessment of platelet functional activity by turbidimetric aggregometry | baseline |
| Impedance of platelet aggregates | Assessment of platelet functional activity by impedance aggregometry |
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Inclusion Criteria:
Exclusion Criteria:
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Healthy pediatric volunteers aged 1 to 17. Pediatric volunteers aged 1 to 17 with any manifestation of hemorrhagic syndrome.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Ekaterina Shamova, Dr | Contact | +375297520785 | lubakova@gmail.com | |
| Viacheslav Dmitriev, Dr | Contact | +375296592670 | rrchypp@gmail.com |
| Name | Affiliation | Role |
|---|---|---|
| rrchypp@gmail.com Shamova, Dr | the Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus | Study Director |
| Viacheslav Dmitriev, Dr | Belarussian Research Center for Pediatric Oncology, Hematology and Immunology |
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Whole blood
| Platelet aggregation and plasma coagulation | Diagnostic Test | Turbodimetric platelet aggregation test, impedance platelet aggregation test. Standard plasma coagulation test (fibrinogen level, prothrombin test, thrombin time, activated partial thromboplastin time (aPTT)). |
|
| baseline |
| Fibrinogen level | Assessment of blood coagulation using Fibrinogen level test | baseline |
| Prothrombin test | Assessment of blood coagulation using Prothrombin test | baseline |
| Thrombin time | Assessment of blood coagulation using Thrombin time test | baseline |
| aPTT | Assessment of blood coagulation using aPTT | baseline |
| ID | Term |
|---|---|
| D016553 | Purpura, Thrombocytopenic, Idiopathic |
| D001791 | Blood Platelet Disorders |
| D006474 | Hemorrhagic Disorders |
| ID | Term |
|---|---|
| D011696 | Purpura, Thrombocytopenic |
| D011693 | Purpura |
| D001778 | Blood Coagulation Disorders |
| D006402 | Hematologic Diseases |
| D006425 | Hemic and Lymphatic Diseases |
| D057049 | Thrombotic Microangiopathies |
| D013921 | Thrombocytopenia |
| D000095542 | Cytopenia |
| D001327 | Autoimmune Diseases |
| D007154 | Immune System Diseases |
| D006470 | Hemorrhage |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D012877 | Skin Manifestations |
| D012816 | Signs and Symptoms |
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| ID | Term |
|---|---|
| D005434 | Flow Cytometry |
| D010974 | Platelet Aggregation |
| ID | Term |
|---|---|
| D002469 | Cell Separation |
| D003584 | Cytological Techniques |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
| D003592 | Cytophotometry |
| D005470 | Fluorometry |
| D008163 | Luminescent Measurements |
| D010783 | Photometry |
| D002623 | Chemistry Techniques, Analytical |
| D008919 | Investigative Techniques |
| D018056 | Hemorheology |
| D001790 | Blood Physiological Phenomena |
| D002943 | Circulatory and Respiratory Physiological Phenomena |
| D015539 | Platelet Activation |
| D006487 | Hemostasis |
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