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| ID | Type | Description | Link |
|---|---|---|---|
| 2021-A02529-32 | Registry Identifier | IDRCB |
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| Name | Class |
|---|---|
| Fondation de France | OTHER |
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The purpose of this study will be to study the association between the level of psychic symptomatic of anorexia nervosa (AN) (intensity of food restriction, symptoms of anxiety and depression) and alteration of host environment symbiosis and the mechanism (dysbiosis of intestinal microbiota, increase of intestinal permeability, immunity alteration and low-grade inflammation).
This is a monocentric study aims to characterise the intestinal microbiota of anorexia nervosa patients with malnutrition, in comparison with the control subject, by DNA sequencing and metagenomic method. The interaction of intetinal microbiota with the host will be studied through the mecanistic studies and various parameters of alteration of symbiosis (intestinal permeability, inflammation) and their association with psychic symptoms of anorexia nervosa.
The objective of this approche is to have not only a descrition of genomic of material of sampling, but also an overview of its potential functioning.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Anorexia patients | Hospitalized anorexia patients with body mass index < 15. |
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| Healthy controls | Health volunteers: 18 years or older with body mass index between 18.5 and 25. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Stool and blood samples | Biological | Stool and blood samples |
|
| Measure | Description | Time Frame |
|---|---|---|
| Eating Disorder | By Eating Disorder Inventory (EDI-3) | at baseline |
| Anxio-depressive symptomatologic assessements by HAD score | By HAD score | at baseline |
| Anxio-depressive symptomatologic assessements by scale of Beck BDI 13 | By scale of Beck BDI 13 | at baseline |
| Anxio-depressive symptomatologic assessements by LSAS Lieboweitz social anxiety scale | By LSAS Lieboweitz social anxiety scale | at baseline |
| Anxio-depressive symptomatologic assessements by MOCI | By Maudsley Obsessions and compulsions inventory (MOCI). | at baseline |
| Anxio-depressive symptomatologic assessements | By HAD score, scale of Beck BDI 13, LSAS Lieboweitz social anxiety scale, Maudsley Obsessions and compulsions inventory (MOCI). | at baseline |
| Physical exercise | By Global physical activity questionnaire (GPAQ) | at baseline |
| Biological parameter | By serum serotonin level | at baseline |
| Measure | Description | Time Frame |
|---|---|---|
| Diversity indice | By index de Sympson et Shannon | at baseline |
| Intestinal permeability | By serum zonulin level | at baseline |
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Inclusion Criteria:
For patients:
For Healthy Volunteers:
Exclusion Criteria:
For patients:
For Healthy Volunteers:
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Hospitalized patients with anorexia nervosa for nutrition rehabilitation in the department of nutrition in Paul Brousse hospital (Villejuif) - APHP.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Mouna HANACHI GUIDOUM, MD | Contact | + 33 6 70 39 42 37 | mouna.hanachi@aphp.fr | |
| Joël DORE | Contact | + 33 1 34 65 27 09 | joel.dore@jouy.inrae.fr |
| Name | Affiliation | Role |
|---|---|---|
| Mouna HANACHI GUIDOUM, MD | Service de Nutrition Clinique, Hôpital Paul Brousse - APHP | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Service de Nutrition Clinique, Hôpital Paul Brousse (APHP) | Recruiting | Villejuif | 94800 | France |
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| ID | Term |
|---|---|
| D000856 | Anorexia Nervosa |
| D064806 | Dysbiosis |
| ID | Term |
|---|---|
| D001068 | Feeding and Eating Disorders |
| D001523 | Mental Disorders |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
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| ID | Term |
|---|---|
| D003672 | Defecation |
| D001800 | Blood Specimen Collection |
| ID | Term |
|---|---|
| D004068 | Digestive System Physiological Phenomena |
| D055688 | Digestive System and Oral Physiological Phenomena |
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
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Stool sample will be collected for all of 2 types of subjects (patients and health volunteers).
| Immunity alteration | By interleukins and CRP level | at baseline |
| Liver function | Cytolyse analysis in serum | at baseline |
| BMI | Calculate of BMI. | at baseline |
| Body composition | By Dexa-Scan. | at baseline |
| Energy expenditure | Calculate of indirect calorimetry and blood parameters | at baseline |
| Intestinal functional disorders | By Francis scale, ,transit with pellets and abdomen x-ray | at baseline |
| Gene and species richness | By 16S rRNA gene sequencing method and Metagenomics | at baseline |
| Circulating mitochondrial DNA copy number | By telemore size and HLA genotyping | at baseline |
| Heart damage | By Left Ventricle Ejection Fraction < 50% structural parameters on ultrasound | at baseline |
| Presence of Autistic symptoms (1) | By Glasgow Sensory Questionnaire GSQ) | at baseline |
| Presence of Autistic symptoms (2) | By The SWedish Eating Assessment for Autism spectrum disorders GSQ) | at baseline |
| EDI | QUESTIONNAIRE EDI or EATING DISORDER INVENTORY (Eating disorder inventory 2nd edition (EDI-2) | at baseline |
| Eating Disorder Diagnostic | By Eating Disorder Diagnostic Scale (EDDS)-DSM-5 VERSION (2015 Wiley Periodicals) | at baseline |
| Eating behaviors assessment | By the Detail and Flexibility Questionnaire (DFlex). | at baseline |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |