The Transcriptomic Study of Thai Patients With Atopic Dermatitis by Tape Strips

The Transcriptomic Study of Thai Patients With Atopic Dermatitis by Tape Strips

This study will be use the tape strip technique to evaluate the skin biomarkers of atopic dermatitis among Thai patients to differentiate clinical phenotype.

The patients will be enrolled in this study if they have been diagnosed with atopic dermatitis. All participants (AD patients and controls) will be evaluated their skin biomarkers by using tape stripping. The tape strips will be applied to the antecubital fossa to collect the epithelial samples. Then RNA was extracted from the tape strips for mRNA profiling to identify the immune and epidermal barrier genes.

Inclusion Criteria:

• Children (age 1-18 years old) with mild atopic dermatitis
• Children (age 1-18 years old) with moderate to severe atopic dermatitis
• Children (age 1-18 years old) with moderate to severe atopic dermatitis and food allergy
• Adult (age 18-60 years old) with atopic dermatitis
• Healthy individuals (1-60 years old)
• Patients with asthma (1-60 years old)

Exclusion Criteria:

• Active skin infections
• Used systemic immunosuppressants within 4 weeks
• Used topical steroids or immunomodulators within 1 week
• Used moisturizers within 12 hours before evaluation