Usage of Oral7 mouth spray along side with the Standard Preventive Advice

Usage of normal saline containing mouth spray along side with the Standard Preventive Advice

The following were the advices given face-to-face to parent and participant and a take home pamphlet was provided:

• Ensure thorough cleaning especially at the gum line and in the areas between the teeth.
• To not share toothbrushes.
• To use toothpaste containing fluoride at least 1000ppm fluoride concentration.
• To store toothbrushes apart to prevent spread of diseases through the bristles.
• To brush teeth at least twice a day, after meals and before bed.
• To brush for as long as two to three minutes.
• Parents would be advised told to supervise the participant's brushing.

Frequency of using the Mouth spray would be two puffs, three times a day that is in the morning after waking up, during tea time and before sleep. A squirt is to be applied to the right/left side of the mouth on the buccal aspect and a squirt on the lingual aspect. Parents will be asked to apply the spray and to supervise children during oral hygiene procedures.

Was measured using the Silness-Loe Plaque Index 1964 from each patient before and after the intervention in both control and case study groups and the mean score and standard deviation was recorded in a table.

Scoring Criteria 0 No plaque

1. A film of plaque adhering to the free gingival margin and adjacent area of the tooth.
2. Moderate accumulation of deposits within the gingival pocket, on the gingival margin and/ or adjacent tooth surface.
3. Abundance of soft matter within the gingival pocket and/or on the tooth and gingival margin.

PIaque Score formula:

Total scores / No of teeth examined

Range of scores :

Excellent : 0 Good : 0.1-0.9 Fair: 1.0-1.9 Poor: 2.0-3.0

Was measured using the Loe and Silness Gingival Index 1963 from each patient before and after the intervention in both control and case study groups and the mean score and standard deviation would be recorded in a table.

Scoring Criteria 0 No inflammation.

1. Mild inflammation, slight change in color, slight edema, no bleeding on probing.
2. Moderate inflammation, moderate glazing, redness, bleeding on probing.
3. Severe inflammation, marked redness and hypertrophy, ulceration, tendency to spontaneous bleeding.

Gingivitis score for the individual would be calculated using a formula as follows :

Sum of indices of teeth / no of teeth examined

These scores was then classified into either a Mild , Moderate or Severe form of Gingivitis as follows :

Mild gingivitis : 0.1-1.0 Moderate gingivitis : 1.1-2.0 Severe gingivitis : 2.1-3.0

The saliva sample was collected from 3 patients each of both case control and study groups before and after the intervention. Saliva samples were collected at the start of the study that is during the delivery of the short dental hygiene educational message, and after 4 weeks. Every child involved was then told to spit in a centrifuge tube to obtain 1-2 ml of total volume. The saliva was then cultured in the lab for saliva bacteria analysis.