Method optimization for the quantification of the NAD metabolome in human whole blood, and evaluation of baseline inter and intra-subject variability.

Whole blood from healthy participants will be collected to optimize analytical method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for the analysis of the NAD metabolome in biological samples. Parameters such as collection volume and anti-coagulants will be investigated for optimal quantification. Moreover, inter- and intra-subject variability of NAD metabolite levels in whole blood will be evaluated. Plasma and urine will also collected for method development and variability measurements.

Inclusion Criteria:

1. Healthy men and women (based on anamnesis),
2. Age between 18 and 55 years,
3. Body Mass Index (BMI) = weight (kg) / height (m)2 between 18.5 to 28 kg/m2.

Exclusion Criteria:

1. Pregnant or lactating women
2. Sick or with a strong cold
3. With vitamin B3 supplementations or under low carbohydrate of ketogenic diet
4. Under prescribed chronic medication, except contraceptive pill.

Healthy volunteers