the vertical dimension of occlusion (VDO) were were determineded through facial appearance observation,thus,the patients were trained to execute bordering protrusive, retrusive and bilateral lateral-protrusive movements wearing the intraoral apparatus and a Gothic Arch Tracing was made, the apex of the Gothic Arch Tracing was defined as the gothic arch position.

the participant were subjected to a low-frequency transcutaneous electrical nerve stimulation (TENS) using the J5 Myotronics for 45 minutes,With the help of TENS, complete relaxation of the muscles were achieved and the mandibular musculature was induced to guide the mandible in the physiologic position,thus using K7 to track the physical mandibular positon which was defined as the neuromuscular position.

All participants were recorded the centric relation registrations according to the technique of the gothic tracing method and the neuromuscular method separately.

according to the method of Ikeda and Kawamura, tmj space measurement in sagittal of the CBCT images were performed

Using the sinus/airway feature of the Dolphin software to reconstruct the upper airway,it included three region: the nasopharynx, oropharynx, and hypopharynx

convert the DICOM files into the exocad software and analyse the difference of the mandibular position determined by the technique of the gothic tracing method and the neuromuscular method separately.