An observational analytic retrospective study was conduct using cross-sectional study. The subject were oocytes from in-vitro fertilization procedures using the ICSI method at the assisted reproductive technology clinic in a private hospital in Bandung for the period 2017 - 2019. Three variables were oocyte denudation time, ICSI time and embryo quality collected from samples that met the research criteria.

This study analyses the different oocyte denudation time after ICSI on all embryos generated during IVF procedures between 1 Januaryr 2017 and 31 December 2019. All oocytes arer categorised on how many hours their denudation times were and these were then assessed for their qualities.

Inclusion Criteria:

• oocytes collected from in-vitro fertilisation procedures involving intracytoplasmic sperm injection

Exclusion Criteria:

• oocytes from patients >40 years old
• oocytes from primary infertility >5 years
• endometriosis
• polycystic ovarian syndrome

Oocytes are derived from patients enrolled in IVF

Anonymised data on oocytes and IVF procedures are only available upon reasonable written request to the authors team.