Complex Ocular Infection, Optimization of Microbiological Diagnosis

Complex Ocular Infection, Optimization of Microbiological Diagnosis

The purpose of this study is to evaluate the impact of different technique to optimize the microbiological diagnosis of the COI.

• Metagenomic for the endophtalmitis
• Multiplex polymerase chain reaction for corneal abscesses

Microbiological diagnosis of complex ocular infection (COI) (i.e: endophtalmitis and corneal abscess) is a current challenge. Indeed, endophtalmitis are often germ-free because a lack of microbiological diagnosis due to small volume to analyze and a complex site to attain. The microbiological etiologies of corneal abscesses are more frequently identified.

Since few years, new molecular tools are developed in infectious diseases to optimizing the microbiological diagnosis. The investigators implemented these techniques in our hospital to optimize the microbiological diagnosis of complex ocular infection (COI). Thus, endophtalmitis benefit, when the volume of the ocular sample is sufficient, of molecular techniques (16s PCR and metagenomic shotgun). Corneal abscesses could shortly benefit of multiplex PCR in order to reduce the time to diagnosis.

The impact and accuracy of these techniques is unknown.

Inclusion Criteria:

• Adult patient

• Patient presenting or having presented a clinical suspicion of complex ocular infection requiring a sample for microbiological diagnosis:

  • Corneal abscess requiring hospitalization
  • Any suspicion of endogenous or exogenous endophthalmitis.

• Patient not opposed to participating in the research

Exclusion Criteria:

• Patient under guardianship or curatorship
• Pregnant women