This study investigated the efficacy of adipose derived stem cell conditioned media (ADSC-CM) combined with minoxidil for hair regeneration therapy in male AGA.

This study was a double-blind, randomized clinical trial (RCT). Two concentrations of ADSC-CM were utilized: concentrated and non-concentrated ADSC-CM. The scalp was divided vertically in half; one side was injected with 2 ml of ADSC-CM, while the control side received 2 ml of NaCl 0,9%. After the injection, patients applied topical 5% minoxidil twice daily. Clinical improvements were assessed using photographic evaluation and trichoscan every two weeks.

Inclusion Criteria:

• males aged 25-58 years old
• Hamilton-Norwood criteria grades II to VI
• cessation of anti-androgens and minoxidil treatment for at least one month

Exclusion Criteria:

• patients with hair loss other than AGA, such as alopecia areata, chemotherapy-induced alopecia, autoimmune-induced alopecia, and scalp malignancies;
• patients receiving 5-alpha reductase inhibitor therapy;
• patients who had received growth factor treatment (platelet-rich plasma or microneedling) for at least six months prior to the study; and
• history of hypertrophic scars or blood clotting disorders.