Effect of a 4-week Post-exercise Sauna Bathing on Targete... | NCT05277597

Status Module

Oversight Module

Description Module

Design Module

Interventional Study Design

Biospecimen

No data available

No data is available for this block.

Arms/Interventions Module

Arm Groups

Label	Type	Description	Intervention Names
The group (ET+S )	Experimental	Participants exercised 60 minutes, 3 times per week, on a bicycle ergometer followed by a 30-minute dry Finish sauna treatment.	Diagnostic Test: The exercise 60 minutes, 3 times per week, on a bicycle ergometer followed by a 30-minute dry Finish sauna treatment.
The group (ET)	Active Comparator	Participants exercised 60 minutes, 3 times per week	Diagnostic Test: The same exercise training program without the sauna treatments

Interventions

Name	Type	Description	Arm Group Labels	Other Names
The exercise 60 minutes, 3 times per week, on a bicycle ergometer followed by a 30-minute dry Finish sauna treatment.	Diagnostic Test	Participants in the group ET+S (n=8) exercised 60 minutes, 3 times per week, on a bicycle ergometer followed by a 30-minute dry Finish sauna treatment.Blood and stool samples were collected before and after the 4-week training program.

Outcomes Module

Primary Outcomes

Measure	Description	Time Frame
Change from abundance of Faecalibacterium prausnitzi of the genus Faecalibacterium, Akkermansia muciniphila of the genus Akkermansia, Bifidobacterium spp. of the genus Actinobacteria and Bacteroides spp. of the genus Bacteroidetes at 4 weeks.	Bacterial DNA was isolated from stool samples using the QIAamp Fast DNA Stool Mini Kit (QIAGEN, Danish). The anaerobic bacteria were determined by Real-Time PCR with appropriate primers (ThermoFisher Scientific, USA).The results of quantitative bacterial analysis were converted to the decimal logarithm (Log10). The entire Real-Time PCR methodology was developed and validated by the Institute of Microecology in Herborn, Germany	baseline and immediately after the intervention
Change from the concentrations of sIgA (marker of mucosal immunity), and zonulin (marker of intestinal permeability) in stool at 4 weeks.	Secretory immunoglobulin A concentrations in stool samples were determined with the Secretory IgA test (ImmuChrom GmbH, Heppenheim, Germany). Zonulin concentrations were assessed using the IKD Zonulin ELISA Kit (Immunodiagnostik AG, Bensheim, Germany).	baseline and immediately after the intervention
Change from the concentration of high-sensitivity C-reactive protein (hsCRP) at 4 weeks.	The concentration of hsCRP was measured by immunoenzymatic assay using a commercially available kit (DRG International Inc., Springfield Township, NJ, USA).	baseline and immediately after the intervention

Secondary Outcomes

Measure	Description	Time Frame
Change from the white blood cell counts (WBC) and subsets at 4 weeks.	Blood samples (approx. 2 ml) were taken from the antecubital vein.Complete blood count indices were determined by flow cytometry with a Synergy 2 SIAFRT analyser (Bio Tek, Winooski, VT, USA).	baseline and immediately after the intervention

Eligibility Module

Name	Affiliation	Role
Joanna Karolkiewicz, Prof.	Poznan University of Physical Education	Principal Investigator

Facility	Status	City	State	ZIP	Country	Contacts
Tomasz Cisoń		Nowy Sącz		33-300	Poland

References Module

Citations

PubMed Identifier	Type	Citation	Retractions
35710395	Derived	Karolkiewicz J, Nieman DC, Cison T, Szurkowska J, Galecka M, Sitkowski D, Szygula Z. No effects of a 4-week post-exercise sauna bathing on targeted gut microbiota and intestinal barrier function, and hsCRP in healthy men: a pilot randomized controlled trial. BMC Sports Sci Med Rehabil. 2022 Jun 16;14(1):107. doi: 10.1186/s13102-022-00497-z.