Assessment of de novo lipogenesis non-invasively in exhaled breath in overnight-fasted humans by triggering feeding response by deuterium resolved mass spectrometry.

1. Healthy participants have a good nights sleep whereas they don't eat during the night and until measurements.
2. They come to the lab and make first baseline exhalation sets. An exhalation set consists of 6 exhalations in Mass Spectrometer positive mode and the same in negative mode. One exhalation set takes around 7minutes from start to finish.
3. Participants consume a single oral dose of dose of 70% 2H2O at 2 g/kg body-water.
4. Participants execute several exhalation sets over time.
5. Participants consume 300g of 'Excellence Joghurt Nature gezuckert.
6. Participants execute several exhalation sets over time.
7. After around 4.5hours with regular exhalation sets taking place, the trial is concluded.

Variation of signal intensity of deuterated exhaled metabolites across the approximately lasting 4.5 hours trial