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To evaluate the effects of blood preservation solution III (sodium citrate, sodium dihydrogen phosphate, sodium citrate, glucose and adenine) in autologous blood recovery and storage bags on the metabolic morphological functions and characteristics of red blood cells in patients undergoing cardiac surgery supported by cardiopulmonary bypass
Blood preservation solution III is a blood protective solution clinically used to store whole blood and red blood cells. It is a sterilized aqueous solution composed of sodium citrate dihydrogen phosphate sodium citrate glucose and adenine, in which glucose adenine can provide the required energy for stored RBC. Citric acid/sodium citrate buffer pair can adjust the pH of RBC suspension; Sodium dihydrogen phosphate can prevent RBC aggregation, provide phosphate for RBC energy metabolism, and slow down the decline rate of 2,3-DPG. Blood preservation solution III can reduce RBC destruction and protect its function. Therefore, this study aims to explore the effect of adding blood preservation solution III in blood storage bags on CPB Influence of autologous blood recovery RBC during cardiac surgery.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| the blood storage bag contains blood preservation solution III | Experimental | a blood storage bag containing blood preservation solution III preserves the washed RBC |
|
| empty blood bag to store washed RBC | No Intervention | an empty blood storage bag preserves the washed RBC |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| blood preservation solution III | Combination Product | Blood bag added blood preservation solution III |
|
| Measure | Description | Time Frame |
|---|---|---|
| hemoglobin and free hemoglobin concentrations | hemoglobin and free hemoglobin concentrations in both groups | up to 48 hours |
| Measure | Description | Time Frame |
|---|---|---|
| RBC morphology | erythrocyte morphology was observed by Rayner's staining and examined under microscope | autologous blood transfusion immediately, 24 hours, 48 hours |
| Adenosine acid (ATP ADP AMP) content in RBC |
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Inclusion Criteria:
Elective median incision for cardiac surgery under CPB ASAâ… ~â…¢ Intraoperative blood recovery is pollution-free No blood system diseases, no red blood cell related diseases Preoperative blood routine examination showed no obvious abnormalities in blood coagulation function and biochemistry
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Mingxia Liu | Contact | 17318531819 | liu17318531819@163.com | |
| Min Yan | Contact |
| Name | Affiliation | Role |
|---|---|---|
| Min Yan | 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| The Second Affiliated Hospital of Zhejiang University anesthesiology department | Hangzhou | Zhejiang | 310000 | China |
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the clarified supernatants were used for ATP, ADP, and AMP measurements with kits
| autologous blood transfusion immediately, 24 hours, 48 hours |
| 2, 3-diphosphate (2,3-DPG) concentration | the cellular 2,3-DPG was analyzed fluorometrically with a kit | autologous blood transfusion immediately, 24 hours, 48 hours |