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This study was designed to investigate the efficacy of flow cytometry to accurately identify between normal and cancer cells in colon epithelium in humans diagnosed with colorectal cancer.
Tissue samples from normal and cancerous colon epithelium are collected intraoperatively following standard colectomy and analyzed blindly by flow cytometry, which reports, in less than 10 min, on the origin of the sample based in the estimation of DNA and tumor index.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| normal colorectal epithelium | Tissue samples from normal colorectal epithelium are collected during colectomy in every colorectal cancer patient |
| |
| cancer colorectal epithelium | Tissue samples from colorectal cancer are collected during colectomy in every colorectal cancer patient |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| flow cytometry | Diagnostic Test | evaluation of intraoperative flow cytometry in detecting cancer cells in colorectal epithelium |
|
| Measure | Description | Time Frame |
|---|---|---|
| Accuracy of intraoperative Flow Cytometry for cancer cell identification in colorectal cancer. | Samples from cancer and normal colon epithelium are prospectively collected intraoperatively and assessed with intraoperative Flow Cytometry. Following comparative DNA content and phenotypic analysis, the receiver operating characteristic (ROC) curve analysis is used to evaluate the performance of intraoperative Flow Cytometry to distinguish between normal and cancer tissue. The optimal cut-off value is determined to delineate the status of tumor tissue, resulting in the highest sensitivity and specificity and hence the optimal accuracy. | 2017-2021 |
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Inclusion Criteria:
biopsy proven colorectal cancer signed informed consent for participation
Exclusion Criteria:
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patients diagnosed with colorectal cancer and being treated by standard colectomy or/and neoadjuvant theraphy according to current guidelines.
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University Hospital of Ioannina | Ioannina | Epirus | 45500 | Greece |
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| ID | Term |
|---|---|
| D015179 | Colorectal Neoplasms |
| ID | Term |
|---|---|
| D007414 | Intestinal Neoplasms |
| D005770 | Gastrointestinal Neoplasms |
| D004067 | Digestive System Neoplasms |
| D009371 | Neoplasms by Site |
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| ID | Term |
|---|---|
| D005434 | Flow Cytometry |
| ID | Term |
|---|---|
| D002469 | Cell Separation |
| D003584 | Cytological Techniques |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
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tissue samples from normal and cancer epithelium collected following colectomy
| D009369 | Neoplasms |
| D004066 | Digestive System Diseases |
| D005767 | Gastrointestinal Diseases |
| D003108 | Colonic Diseases |
| D007410 | Intestinal Diseases |
| D012002 | Rectal Diseases |
| D003933 | Diagnosis |
| D003592 | Cytophotometry |
| D005470 | Fluorometry |
| D008163 | Luminescent Measurements |
| D010783 | Photometry |
| D002623 | Chemistry Techniques, Analytical |
| D008919 | Investigative Techniques |