40 patients for tonsils

40 patients for appendixes)

To identify microchimeric cells of maternal origin in lymphoid in young males cytogenetic FISH staining, coupled with MS-PCR, to identify microchimeric cells.

To identify microchimeric cells of maternal origin in digestive tissues in young males cytogenetic FISH staining, coupled with MS-PCR, to identify microchimeric cells.

Quantifying the microchimeric cells : Cell quantification will be assessed through FISH staining (automated cell counting) and qPCR

locating the cells in tissues : Appendix histology slides will be analysed after FISH staining to locate the microchimeric cells

trying to find a correlation between breastfeeding status : Microchimeric cell quantification will be confronted to the breastfeeding status in infancy (collected during patient inclusion) to validate a correlation