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With this experiment, the experimenter wish evaluate the role of TRPA1 on non-histaminergic itch induced by BAM8-22
With this experiment, the experimenter wish evaluate the role of TRPA1 on non-histaminergic itch induced by BAM8-22 2 (Bovine Adrenal Medulla), which is found naturally in the human body and which has proved to evoke itch. To evaluate the role of TRPA1 it will be used a TRPA1 antagonist (A-967079)
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| A-967079 Injections + BAM8-22 | Experimental |
| |
| A-967079 Injections and vehicle | Experimental |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| A-967079 and BAM8-22 application | Drug | A-967079 solution (in PBS 0.5 mg/ml, subcutaneous injection of 0.1ml) and BAM8-22 with inactivate cowhage spicules 20 µl of BAM8-22 will be applied to a previously determined area on the volar forearm using inactivate cowhage spicules |
| Measure | Description | Time Frame |
|---|---|---|
| superficial blood perfusion | is measured by a speckle contrast imager (FLPI, Moor Instruments, England) | change from baseline, up to 10 minutes after the application |
| Warm Detection Threshold, and Heat Pain threshold | he tests for thermal sensation will all be performed using a PATHWAY ATS (Medoc Ltd, Israel) thermal sensory testing device | change from baseline, up to 10 minutes after the application |
| Cold Detection Threshold and Cold Pain threshold | the tests for thermal sensation will all be performed using a PATHWAY ATS (Medoc Ltd, Israel) thermal sensory testing device | change from baseline, up to 10 minutes after the application |
| Pain supra-threshold heat Stimuli | the tests for thermal sensation will all be performed using a PATHWAY ATS (Medoc Ltd, Israel) thermal sensory testing device | change from baseline, up to 10 minutes after the application |
| Alloknesis | s measured by using a mildy pruritic, non-painful von Frey filaments of a predetermined intensity (generally 5-30 milliNewton of force) | change from baseline, up to 10 minutes after the application |
| mechanical pain threshold and sensitivity | is performed using a pin-prick set (Aalborg University). The set consists of 8 needles each having a diameter of 0,6 mm and different force applications | change from baseline, up to 10 minutes after the application |
| Measure | Description | Time Frame |
|---|---|---|
| pain rating | the subject will rate the pain for 10 minutes by using a visual analog scale (VAS) on a tablet. this scale goes from 0 to 100. 0 indicates "no pain" and 100 indicates "worst imaginable pain" | change from baseline, up to 10 minutes after the application |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Giulia Erica Aliotta | Aalborg | 9000 | Denmark |
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| ID | Term |
|---|---|
| D011537 | Pruritus |
| ID | Term |
|---|---|
| D012871 | Skin Diseases |
| D017437 | Skin and Connective Tissue Diseases |
| D012877 | Skin Manifestations |
| D012816 | Signs and Symptoms |
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| ID | Term |
|---|---|
| C560402 | A 967079 |
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| A-967079 and BAM8-22 application after 5 minutes. | Drug | A-967079 solution (in PBS 0.5 mg/ml, subcutaneous injection of 0.1ml). BAM8-22 with inactivate cowhage spicules 20 µl of BAM8-22 will be applied 5 minutes after to a previously determined area on the volar forearm using inactivate cowhage spicules |
|
| A-967079 application. | Drug | A-967079 solution (in PBS 0.5 mg/ml, subcutaneous injection of 0.1ml) will be applied to a previously determined area on the volar forearm |
|
| Vehicle and BAM8-22 application. | Drug | Vehicle (in PBS, subcutaneous injection of 0.1ml) and BAM8-22 with inactivate cowhage spicules 20 µl of BAM8-22 will be applied to a previously determined area on the volar forearm using inactivate cowhage spicules |
|
| itch rating |
the subject will rate the itch for 10 minutes by using a visual analog scale (VAS) on a tablet. this scale goes from 0 to 100. 0 indicates "no itch" and 100 indicates "worst imaginable itch" |
| change from baseline, up to 10 minutes after the application |
| D013568 | Pathological Conditions, Signs and Symptoms |