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Human cognitive function is affected by age-related changes, with some areas beginning to decline in mid-adulthood and worsening with age. However, there is evidence that dietary interventions or the incorporation of certain healthy foods or nutrients, into the diet can have protective effects against cognitive decline. These foods include nutrients such as polyunsaturated fats, vitamins E and C, and polyphenols. Pecans are a rich source of polyunsaturated fatty acids, antioxidants (including polyphenols), and vitamin E. Pecans contain more total phenols than any other tree nut suggesting that they may be an ideal bioactive food to enhance cognitive performance; however, the relationship between pecan consumption and cognitive functioning has never been assessed. The overall goal behind this research is to determine the relationship between antioxidant-rich pecans and cognitive functioning in a postprandial state.
This trial will be a double-blinded, randomized, cross-over design in humans. There will be two study visits for two different test meals administered in random order. The two testing visits (v1 and v2) include blood draws and repetitions of the cognitive battery after consuming one of the two different test meals containing 1% milk, Nesquik, and either: 1) pecans, or 2) heavy whipping cream. There will be a 6-8 day washout period between each study visit.
Hypothesis: Investigators hypothesize that the pecan-enriched meal will 1.) improve performance on select measures of postprandial cognitive functioning (such as executive functioning, memory, learning, attention, and processing speed) 2.) improve postprandial markers of glycemic control, inflammation, antioxidant capacity, coagulation potential, and 3.) present a similar suppression of subjective appetite compared to the isocaloric, control meal that does not contain pecans.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Pecan Breakfast Shake | Experimental | Participants will be given a breakfast shake consisting primarily of pecans, and 1% milk. |
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| Cream Breakfast Shake | Active Comparator | Participants will be given a breakfast shake consisting primarily of heavy whipping cream. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Pecan Breakfast Shake | Other | The pecan breakfast shake contains 68 grams of blended raw pecans. Participants will consume this shake at either visit 1 or visit 2 depending on randomization procedures. |
| Measure | Description | Time Frame |
|---|---|---|
| Change in Alphabetic Working Memory, Choice Reaction Time, Digit Vigilance, N-back test, Word Recognition, Rapid Visual Information Processing, Picture Recognition, Immediate Word Recall, and Delayed Word Recall | Measured by Computerized Mental Performance Assessment System (COMPASS) computed scores of percent accuracy (total overall target stimuli/novel stimuli) Brain, Performance, and Nutrition Research Centre, Northumbria University | Change from baseline to 4 hours postprandially |
| Change in Serial Subtractions, Immediate Word Recall, and Delayed Word Recall. | Measured by Computerized Mental Performance Assessment System (COMPASS) computed scores of the number of responses (total correct responses/error responses) Brain, Performance, and Nutrition Research Centre, Northumbria University | Change from baseline to 4 hours postprandially |
| Change in Alphabetic Working Memory, Choice Reaction Time, Digit Vigilance, N-back, Picture Recognition, Rapid Visual Information Processing, Word Recognition. | Measured by Computerized Mental Performance Assessment System (COMPASS) computed scores of reaction time (msec) (overall, correct response, target stimuli, novel stimuli, number of false alarms, and number of missed sequences) Brain, Performance, and Nutrition Research Centre, Northumbria University | Change from baseline to 4 hours postprandially |
| Measure | Description | Time Frame |
|---|---|---|
| Change in glucose and triglycerides | Blood samples will be collected to measure Glucose (mg/dL), Triglycerides (mg/dL) | Change from baseline to 4 hours postprandially |
| Change in physiologic measures of appetite |
| Measure | Description | Time Frame |
|---|---|---|
| Change in subjective measures of motivation | Motivation Visual Analog Scale (VAS) (mm). The range of scores on the continuous VAS is 0-100 mm. Zero represents no motivation while 100 represents the greatest feeling of this outcome. | Change from baseline to 4 hours postprandially |
| Change in subjective measures of alertness, stress, and tranquility |
Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Jamie A Cooper, PhD | University of Georgia | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Georgia- Department of Foods and Nutrition | Athens | Georgia | 30605 | United States |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 39945748 | Derived | Guadagni AJ, Prater MC, Paton CM, Cooper JA. Cognitive function in response to a pecan-enriched meal: a randomized, double-blind, cross-over study in healthy adults. Nutr Neurosci. 2025 Sep;28(9):1075-1092. doi: 10.1080/1028415X.2025.2461018. Epub 2025 Feb 13. | |
| 38971424 | Derived | Prater MC, Guadagni AJ, Cooper JA. Postprandial appetite responses to a pecan enriched meal: A randomized crossover trial. Appetite. 2024 Oct 1;201:107598. doi: 10.1016/j.appet.2024.107598. Epub 2024 Jul 4. |
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The plan is to share group averages through publication.
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This trial will be a double-blinded, randomized, cross-over design. There will be 2 testing visits in which each participant will be randomized to receive either the control (cream) shake or the pecan shake at visit 1; they will receive the opposing shake at visit 2. There will be a 6-8 day washout period between visit 1 and visit 2.
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Involved researchers and participants are blinded to which breakfast shake they are administering and/or receiving.
| Cream Breakfast Shake | Other | The cream breakfast shake contains 138 grams of heavy whipping cream. Participants will consume this shake at either visit 1 or visit 2 depending on randomization procedures. |
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Blood samples will be collected to measure Peptide YY (PYY) (pg/mL), Cholecystokinin (CCK) (pg/mL), Ghrelin (pg/mL), Glucagon-like Peptide 1 (GLP-1) (pg/mL), Gastric Inhibitory Peptide (GIP) (pg/mL), pancreatic peptide (PP) (pg/mL).
| Change from baseline to 4 hours postprandially |
| Change in lipid peroxidation | Malondialdehyde (MDA) (uM) measured via Thiobarbituric acid reactive substances (TBARS) assay. | Change from baseline to 4 hours postprandially |
| Change in insulin | Insulin (uU/mL) | Change from baseline to 4 hours postprandially |
| Change in non-esterified free fatty acids (NEFA) | NEFA (mEq/L) | Change from baseline to 4 hours postprandially |
| Change in angiopoietin-like proteins-3 (ANGPTL3), angiopoietin-like-4 (ANGPTL4), and angiopoietin-like-8 (ANGPTL8) | ANGPTL-3 (pg/mL), ANGPTL-4 (pg/mL), ANGPTL-8 (pg/mL) | Change from baseline to 4 hours postprandially |
| Change in subjective measures of appetite | Appetite Visual Analog Scale (VAS) (mm). The range of scores on the continuous VAS is 0-100 mm. Zero represents no hunger, fullness, prospective consumption, and desire to eat, while 100 represents the greatest feeling of these outcomes. | Change from baseline to 4 hours postprandially. Also measured every hour for 10 hours after subject leaves the lab. |
| Change in total antioxidant capacity | Total antioxidant capacity (uM trolox equivalents) measured via Oxygen Radical Absorbance Capacity (ORAC) assay. | Change from baseline to 4 hours postprandially |
| Change in oxidized low density lipoprotein (LDL) | Oxidized Low-Density Lipoprotein (ox-LDL) (mg/dL) | Change from baseline to 4 hours postprandially |
Computerized Mental Performance Assessment System (COMPASS) - Brain, Performance, and Nutrition Research Centre, Northumbria University. Visual Analogue Mood Scales (VAMS) (mm). The range of scores on the continuous VAS is 0-100 mm. Zero represents lesser feeling of alertness, stress, and tranquility, while 100 represents the greatest feeling of these outcomes. |
| Change from baseline to 4 hours postprandially |
| Change in subjective degree of sleepiness | Stanford Sleepiness Scale, in which the scale is rated from 1 to 7. A rating of 1 indicates feelings of being wide awake/alert/vital/active and a rating of 7 indicates the greatest feelings of sleepiness. | Change from baseline to 4 hours postprandially |