In summary, our study demonstrated the methylation sites of SFRP1 gene promoter in patients with colorectal cancer and adenoma and found SFRP1_16_17_18 CpG site was good performance as a diagnostic marker of colorectal cancer.

Inclusion Criteria:

• consecutive adult patients undergoing an outpatient endoscopy and histopathologically diagnosed as colorectal cancer or adenoma. Also, healthy individuals were invited to be enrolled.

Exclusion Criteria:

• Lynch syndrome
• Familial adenomatous polyposis
• MUTYH associated polyposis
• Peutz-Jeghers syndrome
• Juvenile polyposis syndrome
• Serrated polyposis syndrome

consecutive adult patients undergoing an outpatient endoscopy