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The purpose of this study is to evaluate if the anesthetics propofol and desflurane can damage DNA according to comet essay in patients submitted to lumbar disc surgery.
It is expected that DNA damage information provide the possible genotoxic effects of inhalational and intravenous anesthetics and contribute to the establishment of strategies aimed at reducing the risks to exposed patients.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Desflurane Group | Experimental | Desflurane 1,0 minimum alveolar concentration will be administered during anesthesia maintenance |
|
| Propofol Group | Experimental | Propofol 6 mg//kg/hour will be administered during anesthesia maintenance |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Anesthetics, General Desflurane Maintenance | Drug | Comparison of dna damage of desflurane maintenance with propofol maintenance according to comet analysis |
|
| Measure | Description | Time Frame |
|---|---|---|
| Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage. | Before anesthesia induction |
| Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage. | 2 hours after anesthesia induction |
| Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage. | postoperative 1st day |
| Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage. | postoperative 5th day |
| Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage. | Before anesthesia induction |
| Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage. |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Melike Korkmaz Toker, Assoc. Prof. | Mugla Sıtkı Kocman University Department of Anesthesiology and reanimation | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Mugla Sitki Kocman University | Muğla | 48000 | Turkey (Türkiye) |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 38734439 | Derived | Toker MK, Altiparmak B, Gursoy G, Uysal AI, Dede G, Gundogdu G, Dodurga Y, Ugur B. Comparison of the genotoxicity of propofol and desflurane using the comet assay in the lymphocytes of patients who underwent lumbar discectomy: A randomized trial. Saudi Med J. 2024 May;45(5):468-475. doi: 10.15537/smj.2024.45.5.20240077. |
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| ID | Term |
|---|---|
| D000777 | Anesthetics |
| ID | Term |
|---|---|
| D002492 | Central Nervous System Depressants |
| D045505 | Physiological Effects of Drugs |
| D020228 | Pharmacologic Actions |
| D020164 | Chemical Actions and Uses |
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| Anesthetics, General Propofol Maintenance | Drug | Comparison of dna damage of propofol maintenance with desflurane maintenance according to comet analysis |
|
| 2 hours after anesthesia induction |
| Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage. | postoperative 1 st day |
| Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage. | postoperative 5th day |
| Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage. | before anesthesia induction |
| Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage. | 2 hours after anesthesia induction |
| Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage. | postoperative 1 st day |
| Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage. | postoperative 5th day |
| D002491 | Central Nervous System Agents |
| D045506 | Therapeutic Uses |