Prevalence of premature ovarian insufficiency is defined as the number of participants in the sample with a diagnosis of premature ovarian insufficiency (or failure) in their medical record clinical encounter, problem list, and/or past medical history or laboratory results in their medical record consistent with this diagnosis (elevated FSH or undetectable AMH) out of the total sample.

Prevalence of hearing loss is defined as the number of participants in the sample formally diagnosed with a hearing loss in their medical record in a clinical encounter, problem list, and/or past medical history or an audiology report consistent with hearing impairment out of the total sample.

Prevalence of obesity is defined as the number of participants in the sample with obesity in their medical record out of the total sample. Obesity in pediatric populations is determined using BMI-for-age and obesity in adult populations is determined using BMI. BMI is calculated as the weight in kilograms divided by the height in meters squared.

Participants will be considered to have obesity if:

• There is a diagnosis of obesity in the medical record in a clinical encounter, problem list, and/or past medical history
• For children 17 years and younger: there is an available growth chart/height/weight/other data necessary to calculate the BMI-for-age and the BMI-for-age is at or above the 95th percentile
• For adults 18 years and older: there is a BMI at or above 30 kg/m2 or the data necessary to calculate BMI and it is at or above 30 kg/m2

Prevalence of metabolic syndrome is defined as the number of participants in the sample with dyslipidemia in their medical record out of the total sample. Dyslipidemia will be defined as:

• Diagnosis of dyslipidemia, hypertriglyceridemia, hypercholesterolemia in a clinical encounter, problem list, and/or past medical history
• Laboratory evidence of elevated total cholesterol, LDL, triglycerides, and/or low HDL for sex and age

Prevalence of fatty liver disease is defined as the number of participants in the sample with a formal diagnosis of fatty liver disease in their medical record out of the total sample. Fatty liver disease diagnosis in the medical record in a clinical encounter, problem list, and/or past medical history may also present as:

• Non-alcoholic fatty liver disease (NAFLD)
• Hepatic steatosis
• Simple fatty liver disease
• Non-alcoholic steatohepatitis

Prevalence of hypertension is defined as the number of participants in the sample with evidence of hypertension in their medical record out of the total sample. Evidence of hypertension includes:

• Formal diagnosis of hypertension in a clinical encounter, problem list, and/or past medical history
• For children under 13 years old: a blood pressure reading at or above the 95th percentile for age, height, and sex
• For children between 13 and 17 years old: a blood pressure reading at or above 130/80 mmHg
• For adults 18 years or older: a blood pressure reading at or above 140/90 mmHg