Fluorescence is one of the most commonly used research and detection techniques in the field of biomedical science. The characteristics of fluorescent probe directly affect the performance and application of fluorescence analysis and imaging. Aggregation-Caused Quenching has limited the application of traditional fluorescent probes to some extent. This project intends to systematically evaluate the detection efficiency of new methods through the detection of biomarkers in clinical samples and the comparison with the detection methods of traditional biomarkers, so as to provide theoretical and experimental basis for the establishment of fast and simple biomarker detection technologies with new biological probes.

Inclusion Criteria:

• Sign the informed consent form voluntarily.
• Age 18-70 years old.
• High expression (positive) group: The results of multiple laboratory tests indicate that the marker is higher than the normal range.
• Low expression (negative) healthy volunteer group: those with no underlying diseases and no abnormalities in the physical examination.
• Have routine examination to check the remaining body fluid samples, and the body fluid samples are not contaminated.

Exclusion Criteria:

• Accompanied by other inflammations.
• The data of the subject is not complete, or the collection of cases is incomplete due to other reasons.
• The researcher judged that it was not suitable to participate in this research.

NanFang Hospital samples