Single Cell Leukocyte Landscapes and Cardiovascular Risk in Children With Chronic Kidney Disease

Single Cell Leukocyte Landscapes and Cardiovascular Risk in Children With Chronic Kidney Disease

Chronic kidney disease (CKD) is associated with an increased cardiovascular mortality. In particular children with early-onset CKD have a lifelong increased risk to suffer from cardiovascular disease (CVD). Therefore, children with CKD deserve our attention. The immune system in children with CKD is disturbed, exhibiting pro-inflammatory features. Therefore, we aim to learn more about the characteristics of the immune system in early-onset CKD. In this project PBMC of pediatric CKD patients and age-matched healthy controls will be analysed and compared using CITE-Seq as a multimodal scRNAseq phenotyping method. All patients will be clinically characterized to integrate cardiovascular and immunological data.

Inclusion Criteria:

• matching to one of the following groups CKD G3-5: estimated eGFR according to Schwartz formula <60ml/min*1,73m2 (no ESKD) CKD G5 D: patients with ESKD treated with hemodialysis for at least 3 months normal kidney function: CKD G1 or no CKD with eGFR > 90ml/min*1,73m2
• informed consent to participate in this study

Exclusion Criteria:

• body weight of less than 15kg
• acute or chronic inflammatory diseases
• fever
• diabetes
• chronic liver disease
• inflammatory bowel disease or other gastrointestinal disorders (constipation, diarrhea, short bowel syndrome)
• antibiotic prophylaxis or treatment within four weeks prior to recruitment