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Chronic kidney disease (CKD) is associated with an increased cardiovascular mortality. In particular children with early-onset CKD have a lifelong increased risk to suffer from cardiovascular disease (CVD). Therefore, children with CKD deserve our attention. The immune system in children with CKD is disturbed, exhibiting pro-inflammatory features. Therefore, we aim to learn more about the characteristics of the immune system in early-onset CKD. In this project PBMC of pediatric CKD patients and age-matched healthy controls will be analysed and compared using CITE-Seq as a multimodal scRNAseq phenotyping method. All patients will be clinically characterized to integrate cardiovascular and immunological data.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| CKD G3-5 | Patients with CKD and an estimated eGFR < 60ml/min not yet on dialysis |
| |
| End stage kidney disease (ESKD) CKD G5 Hemodialysis | Patients on hemodialysis for at least 3 months |
| |
| Normal kidney function | Patients enrolled with normal kidney function and/or CKD G1 and an estimated eGFR > 90ml/min |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| No intervention | Other | There will be no intervention in the separate goups, as this is an observational study |
|
| Measure | Description | Time Frame |
|---|---|---|
| Immunephenotyping of human PBMC | scRNAseq with Cellular Indexing of Transcriptomes and Epitopes (CITE)-Seq for whole PBMC will be used to gain information on single cell transcriptomes across multiple immune cell populations together with expression levels of classical mmunological surface markers. | at recruitment |
| Measure | Description | Time Frame |
|---|---|---|
| Microbiome sequencing of fecal samples | 16S RNA amplicon sequencing and whole genome shotgun sequencing will be perfomred in fecal samples, collected in RNA/DNA shield. This will allow us insights in composition (abundances) and function of the gut microbiome. | at recruitment |
| Targeted metabolomics of plasma samples |
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Inclusion Criteria:
Exclusion Criteria:
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Only patients treated at the Department of Pediatric Nephrology in Berlin (Charité), Hamburg (UKE) and Leipzig (KfH) will be recruited for this study.
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| Name | Affiliation | Role |
|---|---|---|
| Dominik Müller, MD | Charite University, Berlin, Germany | Study Director |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Department of Pediatric Gastroenterology, Nephrology and Metabolic Diseases, Charité-Universitätsmedizin Berlin | Berlin | 13353 | Germany |
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| ID | Term |
|---|---|
| D051436 | Renal Insufficiency, Chronic |
| D002318 | Cardiovascular Diseases |
| D003141 | Communicable Diseases |
| ID | Term |
|---|---|
| D051437 | Renal Insufficiency |
| D007674 | Kidney Diseases |
| D014570 | Urologic Diseases |
| D052776 | Female Urogenital Diseases |
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Fecal and blood samples will be collected at a single timepoint at study enrollment.
By using liquid-chromatography (LC) and gas-chromatography (GC) mass-spectrometry plasma samples will be analyzed for tryptophan metabolites and short chain fatty acids (absolute quantifiaction in µM). |
| at recruitment |
| Pulse wave velocity | The pulse wave velocity (m/s) will be measured in all recruited patients by usinfg the Mobilograph. Data will be normalized to age. | at recruitment |
| Carotid intima media thickness | The carotid intima media thickness (mm) will be measured using ultrasound imaging of the carotid vessel. Data will be normalized to age. | at recruitment |
| Echocardiography | A basic echocardiography will be conducted evaluating diastolic and systolic, right and left ventricular function parameters. | at recruitment |
| D005261 |
| Female Urogenital Diseases and Pregnancy Complications |
| D000091642 | Urogenital Diseases |
| D052801 | Male Urogenital Diseases |
| D002908 | Chronic Disease |
| D020969 | Disease Attributes |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D007239 | Infections |