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Purpose: To compare the effects of the density gradient centrifugation and microfluidic chip sperm preparation methods on embryo development in a study population with astheno-teratozoospermia.
Materials and Methods: In this prospective study, the semen samples of the patients with astheno-teratozoospermia were divided into two groups for preparation with either the microfluidic or density gradient methods. Selected spermatozoa were used to fertilize the mature sibling oocytes. Semen parameters and embryo development on days 3 and 5 were assessed.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Density gradient method | Experimental | Sperm prepared by density gradient method |
|
| Microfluidic chip | Experimental | Sperm prepared by microfluidic chip method |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Microfluidic sperm sorting chip | Device | Fertile Ultimate® (Koek Biotechnology, Turkey) microfluidic sperm sorting chips |
|
| Measure | Description | Time Frame |
|---|---|---|
| Semen parameters after sperm preparation with either method: semen volume | semen volume (ml) | up to 7 months |
| Semen parameters after sperm preparation with either method: sperm concentration | sperm concentration (106/ml) | up to 7 months |
| Semen parameters after sperm preparation with either method: total motility | total motility (%) | up to 7 months |
| Semen parameters after sperm preparation with either method: progressive motility | progressive motility (%) | up to 7 months |
| Embryo development after sperm preparation with either method | Embryo development and blastocyst formation were evaluated on days 1, 3 and 5 based on the criteria reported by Veeck and Zaninovic, 2003 | up to 7 months |
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Inclusion Criteria:
Exclusion Criteria:
Split semen samples from the same population of infertile men with astheno-teratozoospermia were used to fertilize the mature sibling oocytes.
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| MaltepeU | Istanbul | Turkey (Türkiye) |
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| Density gradient | Device | Density gradient centrifugation |
|
| ID | Term |
|---|---|
| D053627 | Asthenozoospermia |
| ID | Term |
|---|---|
| D007248 | Infertility, Male |
| D005832 | Genital Diseases, Male |
| D000091662 | Genital Diseases |
| D000091642 | Urogenital Diseases |
| D007246 | Infertility |
| D052801 | Male Urogenital Diseases |
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| ID | Term |
|---|---|
| D002499 | Centrifugation, Density Gradient |
| ID | Term |
|---|---|
| D014461 | Ultracentrifugation |
| D002498 | Centrifugation |
| D008919 | Investigative Techniques |
| D002623 | Chemistry Techniques, Analytical |
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