Evaluation of Kisspeptin Glucose-Stimulated Insulin Secretion With Oral Glucose Tolerance Test

Evaluation of Kisspeptin Glucose-Stimulated Insulin Secretion With Oral Glucose Tolerance Test

The goal of this study is to understand how exogenous kisspeptin affects metabolism by evaluating responses to an oral glucose tolerance test

Assignment: Each study subject will serve as their own control. The order of the visits will be randomized.

Delivery of Interventions:

• Prior to the study visit, subjects will undergo a review of their medical history and screening laboratories.

• During the inpatient study, the subjects will

  • Undergo a 16-hour kisspeptin infusion
  • Undergo an oral glucose tolerance test

Inclusion Criteria:

History:

• over the age of 18,
• normal pubertal development
• stable weight for previous three months,
• normal body mass index (BMI between 18.5-25)
• regular menstrual cycles

Physical examination:

• systolic BP < 140 mm Hg, diastolic < 90 mm Hg

Laboratory studies: (per Massachusetts General Hospital reference ranges)

• normal hemoglobin
• hemoglobin A1C < 6.5%
• blood urea nitrogen, creatinine not elevated
• aspartate aminotransferase, alanine aminotransferase < 3x upper limit of normal

Exclusion Criteria:

• active illicit drug use,
• history of a medication reaction requiring emergency medical care,
• difficulty with blood draws.
• history of chronic disease, except well controlled thyroid disease,
• recent use of prescription medications which interfere with metabolism or reproduction (recent = within 5 half-lives of the drug). Use of levothyroxine or seasonal allergy medications is acceptable,
• history of diabetes in a first degree relative,
• use of contraceptive pills, patches or vaginal rings within last 4 weeks.
• hyperlipidemia by fasting lipid panel
• positive serum pregnancy test (for all women)