1. age:year of 25~60;
2. consistent with the 2015 ACR gout diagnostic criteria ,and serum uric acid >420umol/L;
3. The patient are willing to take part in our study.

1. age:year of 25~60;
2. A medical record in our hospital showed that the person is healthy, without key disease;
3. serum uric acid >420umol/L without gout flares.

1. age:year of 25~60;
2. A medical record in our hospital showed that the person is healthy, without key disease;
3. serum uric acid ≤420umol/L.

We will test the specific SNPs that we have selected before, such as rs670, rs671, rs1014290 and so on. The SNPs will be tested by Taqman SNP assay and verified by sequencing.

Feces will be collected in gout, hyperuricemia patients and healthy subjects. ofIntestinal flora will be tested and analysed. The detailed measurement is as follows: Firstly, the DNA of feces will be extracted according to the protocol of Fecal DNA Extraction Kit. Then, the concentration and purity of fecal DNA will be tested by spectrophotometer. Sequencing of the V3-V4 region of the 16S ribosomal RNA gene, building a library and then sequencing the qualified libraries by Illumina Platform(MiSeq). Using the QIIME、UCLUST、SILVA and Kyoto Encyclopedia of Genes and Genomes(KEGG) database to comparison and screen the intestinal bacterial with statistical significance. Operational taxonomic unit (OTU), α-diversity, β-diversity indices and so on of the fecal microbiota will be analyzed in patients with gout and hyperuricemia and normal control.

Feces uric acid level will be tested by uric acid assay kit and we will analyze the differences of feces uric acid level in gout, hyperuricemia patients and healthy subjects.

follow-up the patients with hyperuricemia for at least one year, calculate the incidence of hyperuricemia patients develop to gout.