Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
This is an observational prospective single-center study of 30 patients operated for early-staged non-small cell lung cancer. The main aim is the analysis of molecular profiling of exosome with a sample in tumor-draining vein in order to identify prognostic molecular characteristics associated with cancer recurrence after surgery.
Surgical resection remains the best chance for cure in patients with early-stage non-small-cell lung cancer (NSCLC). Unfortunately, approximately 30-50% of patients will relapse within 5-year of curative surgery despite early TNM-stage. The Identification of prognostic biomarkers of relapse is a necessity. Among them, extracellular vesicles (exosomes) study seems promising in early stage cancers and samples directly in tumor-draining vein to. The pulmonary vein is draining blood directly from the lungs and sampling at this location could provide a higher yield for oncosome. The litterature reports that Navarro et al. are the only team to have link exosome characteristics to oncological outcomes following surgery. The investigators propose to analyze the molecular profiling mediated by these extracellular vesicles in the tumor-draining vein of operated patients.
A peripheral blood sample is recolted before surgery (D-1 or D0). During oncological lung surgery, the pulmonary tumor-drainage vein is first exposed and punctured with a needle prior to subsequent surgical manipulation for resection (D0). A piece of resected tumor will be analyzed for the study (D0). Quantification and size distribution of exosome and molecular cargo of exosome in blood samples (peripheral and pulmonary vein) and tumor are analyzed (DNA sequencing).
Standard clinical and radiological follow-up is then performed and 2-year overall survival and 2-year disease free-survival are checked.
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Blood samples at 2 sites: peripheral vein and tumor-draining vein | Other | D0: surgery (inclusion): oncological lung resection
|
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Blood samples at 2 sites: peripheral vein and tumor-draining vein | Biological | D0: surgery (inclusion): oncological lung resection
|
| Measure | Description | Time Frame |
|---|---|---|
| Evaluate size distribution, concentration and molecular profiling of pulmonary vein exosomes at inclusion | Concentration of extracellular vesicule (million of particule/mL of blood) | 36 months |
| Measure | Description | Time Frame |
|---|---|---|
| Size distribution, molecular profiling of peripheral vein exosome sat inclusion | The size distribution of extracellular vesicules harvested | 36 months |
| Mutations and polymorphism in resected lung cancer |
Not provided
Inclusion Criteria:
- Surgical non-small cell lung cancer with pure solid nodule or part-solid ground glass nodule on CT-scan
Exclusion Criteria:
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Affiliation | Role |
|---|---|---|
| Jérémy Tricard, MD | University Hospital, Limoges | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| CHU de Limoges | Limoges | Limoges | 87042 | France |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 41923119 | Derived | Tricard J, Durand S, Rovini A, Gateau A, Negroni L, Chaunavel A, Chermat A, Jauberteau MO, Conti M, Akil H, Lalloue F. New proteomic signature in circulating extracellular vesicles from tumor-draining and peripheral veins of patients with lung adenocarcinoma. Cancer Cell Int. 2026 Apr 1;26(1):192. doi: 10.1186/s12935-026-04264-9. |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| ID | Term |
|---|---|
| D008175 | Lung Neoplasms |
| D002289 | Carcinoma, Non-Small-Cell Lung |
| ID | Term |
|---|---|
| D012142 | Respiratory Tract Neoplasms |
| D013899 | Thoracic Neoplasms |
| D009371 | Neoplasms by Site |
| D009369 | Neoplasms |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
|
Analyze of the molecular profile in the resected tumor tissus
| 36 months |
| Overall survival | overall survival | 36 months |
| Two-year disease-free survival | Two-year disease-free survival | 24 months |
| D008171 |
| Lung Diseases |
| D012140 | Respiratory Tract Diseases |
| D002283 | Carcinoma, Bronchogenic |
| D001984 | Bronchial Neoplasms |